Protective effect of 3-NP repetitive preconditioning on dopaminergic neurons
- VernacularTitle:3-硝基丙酸多次化学预处理对多巴胺能神经元的保护作用
- Author:
Deng XUE-JUN
1
;
Sun SHENG-GANG
;
Cao XUE-BING
;
Li HONG-GE
;
Liang ZHI-HOU
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
3-NP;
preconditioning;
Parkinson's disease;
c-Jun
- From:
Neuroscience Bulletin
2005;21(4):266-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of 3-NP repetitive preconditioning on dopaminergic neurons.Methods Parkinson' s disease model were made on C57BL mouse with MPTP ( 30 mg/kg). 3-NP were administered to produce preconditioning before MPTP used. The apoptosis ratio and expression of c-Jun in the substantia nigra were assayed. MPP + were used to made parkinson' s disease model in vitro. 3-NP were incubated to produce preconditioning;The fragment of dominant mutant c-JuncDNA carried by eukaryon plasmid pcDNA3 (HA)-jun-dn was transfected into SHSY5Y cells. The expression of c-Jun and the apoptosis ratio of SH-SY5Y cells were assayed. Results In MPTP group,the ratio of apoptosis was increased significantly. After 3-NP preconditioning, apoptosis was decreased significantly and the effect of repetitive preconditioning was better than that of single preconditioning. The expression of c-Jun was increased in MPTP group and no obviously changes were observed after 3-NP single preconditioning, but decreased after 3-NP repetitive preconditioning. In the transfection group, the expression of c-Jun was decreased and the apoptosis ratio was lower than that without transfection group. 3-NP repetitive preconditioning could decrease the expression of c-Jun and apoptosis ratio. Conclusion 3-NP preconditioning exerts protective effect on dopaminergic neurons and the effect of repetitive preconditioning was better than single preconditioning.
