Induction of silencing effect of Alzheimer disease familial Swedish mutant APP by RNA interference
- VernacularTitle:RNA干扰对阿尔茨海默病家族性瑞典型APP突变的沉默作用
- Author:
Qiu XIN
1
;
Pan JI-AN
;
Chen YU
;
Zhang WEI-YING
;
Yang HUA-JING
;
Zhang SU-MING
Author Information
1. Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology
- Keywords:
amyloid precursor protein (APP);
small interference RNA (siRNA);
wild type APP(APP,);
Swedishmutant APP(APPswe)
- From:
Neuroscience Bulletin
2005;21(3):219-223
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To observe the silencing effect of amyloid precursor protein (APP) carrying Swedish mutation of familial Alzheimer disease triggered by small interference RNA (siRNA). Methods We designed one oligonucleotide against Swedish mutant APP(APPswe) which was endogenously expressed by small interference RNA(siRNA) expressing plasmid . In order to observe the silence effect of siRNA on APP, COS-7 cells were transiently co-transfected with APPEGFP recombinant plasmid and siRNA expressing plasmid. The silencing effect of siRNA was determined by the assay of reporter gene-GFP expression levels. Results APPswe expression in COS-7 cells was knocked down by the application of siRNA. APPswesiRNA was more effective in silencing APPswe gene than wild type APP (APPwt) gene. Conclusion APPswe siRNA can effectively silence APPswe gene expression and such effect is allele-specific. Therefore, siRNA directed against APPswe may have therapeutic potential for the treatment of Alzheimer disease.
