Effect of Ginkgo biloba on expression of N-methyl-D-aspartate (NMDA) receptor 1 mRNA on post-ischemia reperfusion injury in the rat brain
- VernacularTitle:银杏叶制剂对大鼠脑缺血再灌注后N-甲基-D-天冬氨酸受体表达的影响及脑保护作用
- Author:
Li XIAO-HONG
1
;
Zhang FENG
;
Zhao MING-XIA
;
Zhang QIU-LING
;
Zhao ZHONG-XIN
Author Information
1. Ji' nan Central Hospital,Shandong University
- Keywords:
apoptosis;
Egb;
cerebral ischemia;
NMDA-receptor;
ischemia-reperfusion
- From:
Neuroscience Bulletin
2005;21(3):210-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between expression of NMDA receptor 1 ( NR1 ) mRNA and neuron apoptosis in rats during focal cerebral ischemia-reperfusion(I/R) ,the effect of Ginkgo biloba extract(Egb) on them and related mechanism. Methods One hundred and forty SD rats were randomized into 3 groups: sham-operated group ( group Ⅰ, n = 20), ischemia-reperfusion group ( MCAO group/group Ⅱ, n = 60) and Egb treatment group ( group Ⅲ, n = 60).The rats were killed at 6, 24, 48, 96 h after reperfusion for TUNEL and in situ hybridization analysis. TUNEL was used to show cell apoptosis. Results Compared with group Ⅰ , the expression of NR1 mRNA and the number of apoptosis cells in I/R brain tissue increased significantly in group Ⅱ. Compared with group Ⅱ , NR1 mRNA expression and cell apoptosis decreased obviously in group Ⅲ. Conclusions The expression of NR1 mRNA in I/R brain tissue increased significantly than that in group I and might be closely related to neuron apoptosis. And Egb can inhibit the expression of NR1 mRNA in ischemic areas and the neuron apoptosis subsequently.
