The effect of sleep deprivation on cognitive and cerebral mitochondrial respiratory function in rats
- VernacularTitle:不同程度睡眠剥夺对大鼠认知和脑线粒体呼吸功能的影响
- Author:
Dou WEI
1
;
Zhao ZHONG-XIN
;
Miao MING-YONG
;
Wang WEN-ZHAO
;
Huang LIU-QING
Author Information
1. Changzheng Hospital,Second Military Medical University
- Keywords:
sleep deprivation;
cognitive function;
mitochondrial respiratory function;
brain;
rat
- From:
Neuroscience Bulletin
2005;21(3):204-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of various degrees of sleep deprivation on cognitive and cerebral mitochondrial respiratory function in rats. Methods Sprague-Dawley rats were subjected to a 10-day sleep deprivation protocol and were divided into 5 groups randomly: allowing sleep for 12 h (control group), 9 h (group1), 6 h( group2), 3 h (group3), 0 h each day (group4). The modified multiple platform method(MMPM) was used to establish sleep deprivation model. The cognitive function was tested by Y-type maze. Mitochondrial respiratory function was measured by Clarktype oxygen electrode. Results The number of error reactions was significantly increased in sleep deprived rats. There were statistically significant differences( P ＜ 0.05 )in the number of error reactions between the control group and the completely deprived(0 h group)or severely deprived(3 h group)rats on each of the study days. The oxygen consumption rate in state 3 respiration was significantly decreased( P ＜ 0.05 )in sleep deprived rats compared with the control group. The oxygen consumption rate in state 4 respiration was significantly decreased(P ＜ 0.05 )only in the completely deprived(0 h group)rats compared with the control group. Conclusion Sleep deprivation, no matter mild or severe, both could have adverse effects on cognitive function and cerebral mitochondrial respiratory function.
