Effects of recombinant ciliary neurotrophic factor on expression of growth associated protein 43 and somatostatin in the related neurons after sciatic nerve repair in rats
- VernacularTitle:大鼠坐骨神经修复后重组睫状神经营养因子对相关神经元生长相关蛋白43、生长抑素表达的影响
- Author:
Xu JIA-JUN
1
;
Chen ER-YU
;
Lu CHANG-LIN
;
He CHENG
Author Information
1. Second Military Medical University
- Keywords:
nerve repair;
neuron;
ciliary neurotrophic factor;
growth associated protein 43;
somatostatin;
rat
- From:
Neuroscience Bulletin
2005;21(3):198-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of recombinant ciliary neurotrophic factor(CNTF) on expression of growth related proteins in related neurons after sciatic nerve repair in rats. Methods After a segment of the sciatic nerve of the rat was removed, the distal and proximal ends were sutured into a recombinant CNTF-containing silicone tube. Growth associated protein 43 ( GAP-43 ) and somatostatin (SOM) in the L4 spinal cord, L4 or L5 spinal ganglia were examined and measured by immunohistochemistry and in situ hybridization using computerized image processing system. Results An increase in the GAP-43 immunoreactivity in the large, medium neurons of spinal anterolateral nucleus was more significant in the CNTF-treated group compared with those in the 0.9% saline (SAL) -treated group, while a decrease in SOM mRNA hybridization signals in those neurons were more significant in the CNTF-treated group. There were no significant differences in the aforementioned immunoreactivity and hybridization signals of spinal ganglia between the CNTF-treated group and the SAL-treated group. Conclusion The results suggest that recombinant CNTF administration up-regulate GAP-43 expression and down-regulate SOM mRNA expression in the related motor neurons, but not sensory neurons, after reconnection of transected sciatic nerve.
