Effect of thrombin on intracellular free calcium in primary cultured hippocampal neurons
- VernacularTitle:凝血酶对原代培养海马神经元游离钙浓度的影响
- Author:
Yang WEN-QIONG
1
;
Sun SHENG-GANG
;
Tong E-TANG
;
Cao XUE-BING
Author Information
1. Union Hospital,Tongji Medical College,Huazhong University of Science and Technology
- Keywords:
thrombin;
hippocampal neurons;
protease activated receptor-1;
intracellular free calcium
- From:
Neuroscience Bulletin
2005;21(3):188-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of thrombin on concentration of intracellular free calcium in primary cultured hippocampal neurons. Methods Calcium ion indicator Fura-2 fluorophotometry was used to measure concentration of Ca2 + of primary cultured hippocampal neurons treated by different doses of thrombin. Results 1-40 U/ml of thrombin increased the levels of free intracellular Ca2+ in primary cultured hippocampal neurons significantly in a dose-response manner. Thrombin receptor activating peptides (TRAP) mimiced thrombin to increase the intracellular free Ca2+ of neurons. Thrombin increased the rest [ Ca2 + ] i when hippocampal neurons were incubated in 1.3 mmol/L Ca2 + medium, did not elevated [ Ca2+ ]i when neurons incubated in 0.0 mmol/L Ca2+ medium. Increasing in intracellular free Ca2+ of neurons caused by thrombin was associated with large influx of Ca2+ outside cells. MK-801 inhibited the effect of thrombin on intracellular free Ca2 + of neurons. Conclusions Thrombin-increased intracellular free Ca2 + of neurons in a dose-dependent manner may be through activating PAR-1, which is associated with activating NMDA receptor to induce large influx of Ca2+ outside cells.
