A preliminary study on the receptor for axon guidance factor Netrin-4
- VernacularTitle:神经突起诱向因子Netrin-4受体的初步研究
- Author:
Yu KE-KE
1
;
Qin SHU-TONG
;
Wang SI-YING
;
Zhou REN-PING
;
Zhang CHENG-GANG
Author Information
1. Beijing Institute of Radiation Medicine
- Keywords:
axon guidance;
receptor;
Netrin-4;
DCC;
UNC5H
- From:
Neuroscience Bulletin
2005;21(3):175-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the receptors for the axon guidance factor Netrin-4 and whether the known Netrin-1 receptors, DCC and UNC5H1, are coreceptors for Netrin-4. Methods The receptor for Netrin-4 was detected by affinitycytochemistry and in situ staining with the alkaline phosphatase (AP) tagged Netrin-4 fusion protein. The dynamic character of the binding between Netrin-4 and its receptor was evaluated through ligand-receptor binding assay and competitive binding assay. Results In situ staining of AP4-Netrin-4 protein bound to COS7 cells transiently transfected with eukaryotic expression coding DCC and UNC5H1 showed that the Netrin-4 could bind to the transfected cells. Competitive binding assays showed that Netrin-1 could compete with Netrin-4 for the same receptors. Conclusion DCC and UNC5H1 are coreceptors for Netrin-4. However, because the binding affinity of Netrin-4 with its receptor was lower than that of Netrin-1,it could be inferred that there should exist unique receptor(s) for Netrin-4 other than DCC and UNC5H1.
