Dopamine and acetylcholine inhibit the expression of iNOS in primary glia challenged by LPS
- VernacularTitle:多巴胺和乙酰胆碱抑制脂多糖刺激原代胶质细胞iNOS的表达
- Author:
Liu JIA-FU
1
;
Liu ZHEN-GUO
;
Zhou HAI-YAN
;
Chen SHENG-DI
;
Lu GUO-QIANG
;
Liang LIANG
Author Information
1. 上海第二医科大学
- Keywords:
dopamine;
acetylcholine;
primary glia;
LPS;
iNOS
- From:
Neuroscience Bulletin
2005;21(2):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dopamine and acetylcholine on the expression of iNOS in rat primary glia stimulated with the bacterial endotoxin lipopolysaccharide (LPS). Methods We cultured the rat primary glia, pretreated by dopamine and acetylcholine for 30 min, then challenged by LPS for 12 h and 24 h, isolated total RNA for RTPCR , did cell immunohistochemisry, respectively to observe the change of iNOS at transcription and translation levels,measured nitrite in the culture medium using Griess method to assess endogenous synthesis of NO. Results Acetylcholine had no effect on the transcription of iNOS, otherwise dopamine could inhibit it, both of dopamine and acetylcholine could reduce the expression of iNOS protein and the production of NO. Conclusion dopamine and acetylcholine inhibited the expression of iNOS in primary glia challenged by LPS in different ways.
