Correlation between expression of glioma bradykinin B2 receptor and pathological grade of glioma
- VernacularTitle:神经胶质瘤中缓激肽B2受体的表达水平与病理级别的相关性
- Author:
Zhao YI-SONG
1
;
Xue YI-XUE
;
Liu YUN-HUI
;
Fu WEI
;
Jiang NAI-JIA
;
An PING
;
Wang PING
;
Yang ZHI-HANG
;
Wang YU-QIN
Author Information
1. 中国医科大学
- Keywords:
blood-brain barrier;
bradykinin;
B2 receptor;
glioma;
pathological grade
- From:
Neuroscience Bulletin
2005;21(2):135-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the correlation between the expressing level of bradykinin B2receptor and pathological grade of gliomas, and supply experimental basis for clinical application of bradykinin or its analog. Methods The clinicopathologic findings were diagnosed by reviewing all hematoxylin and eosin (H&E) stained tissue sections. To determine the expressing level of bradykinin B2 receptor in glioma, immunohistochemistry and Western blot methods were used.Results Results of H&E staining: In 26 cases of glioma there were 9 cases of grade Ⅰ, 9 cases of grade Ⅱ, 8 cases of grade Ⅲ, and 0 case of grade Ⅳ. Bradykinin B2 receptor localized on tumor cells while the cells of edematous band at the edge of glioma did not express B2 receptor. Results of Western blot: grade Ⅰ and grade Ⅱ (39480.88 ± 5119.96 vs 51354.25 ±6168.77, n = 8, t = 2. 447, P ＜ 0.05); grade Ⅰ and grade Ⅲ (39480.88 ± 5119.96 vs 73032.13 ±8802.71, n = 8, t = 7. 710, P ＜ 0. 001 ); grade Ⅱ and grade Ⅲ (51354.25 ± 6168.77 vs 73032.13 ± 8802.71, n = 8,t = 5. 704, P ＜ 0. 001 ). There was a positive correlation between the expressing level of bradykinin B2 receptor and the the pathological grade of glioma (r =0. 895, P ＜0.001) as grade Ⅰ＜grade Ⅱ＜grade Ⅲ. Results of immunohistochemistry:grade Ⅰ and grade Ⅱ (3.54% 1.51% vs 8.47% ±3.45%, n=8, t=3.698, P＜0.01); grade Ⅰ and grade Ⅲ (3.54%±1.51% vs 15.94% ±1.68%, n=8, t=15.562, P＜0.001); grade Ⅱ and grade Ⅲ (8.47% ±3.45% vs 15.94%± 1.68% , n = 8, t = 5. 505, P ＜ 0. 001 ). There was a positive correlation between the positive position area ratio of bradykinin B2receptor and the pathological grade of glioma (r = 0. 878, P ＜0. 001 ) as grade Ⅰ＜ grade Ⅱ＜ grade Ⅲ. Conclusion There was a positive correlation between the expressing level of bradykinin B2 receptor and the pathological grade of glioma. This may be the reason of the diverse cure effects of the increase of blood-tumor barrier permeability induced by bradykinin and its analog.
