An investigation on the relations between synchronized oscillations and epileptogenesis induced by repeatedly delivering tetanus into the hippocampus and medial temporal neocortex in rats
- VernacularTitle:强直电刺激大鼠海马、中部颞叶新皮质诱发癫癇模型中电振荡与癫癇发生的关系研究
- Author:
Dan HAN
1
;
Xian-Rong ZHANG
;
Yue-Feng TANG
;
Mai-Li LIU
;
Wei-Ze LIU
;
Fang YANG
Author Information
1. 湖北医科大学
- Keywords:
oscillations;
epileptogenesis;
hippocampus;
medial temporal neocortex;
rat
- From:
Neuroscience Bulletin
2000;16(2):108-114
- CountryChina
- Language:Chinese
-
Abstract:
To study the functional significance of oscillations in epileptogenesis we observed the relations between synchronized oscillations and epileptic events at the level of EEG,depth electrographes,local neural networks and single neuron in animal experiments in vivo or in vitro.Works were done on three models of epilepsy:kindling model on 400 μm thick horizontal rat brain slices containing the hippocampus(HPC) and entorhinal cortex(EC),acute and chronic epilepsy model in vivo.Tetanus(60 Hz,2 s,0.4～0.6 mA)was used to induce epilepsy events.The results showed that ①In 32.5%(13/40) brain slices both intracellular and extracellular recordings displayed membrane potential oscillations in CA1 neuron at 3～100 Hz induced by electric stimulus into Schaeffer collaterals.These oscillations at 7 Hz were related to synchronous field epileptiform events and promote its conversion.②In acute epilepsy model(n=10) spontaneous oscillations in the HPC at 4～7 Hz or in the medial temporal neocortex (MTNC) at 20 Hz were activated by tetanus into the MTNC or the HPC,respectively.The increased field oscillations could transfer into associated epilepsy events.③In the epileptic rats,the amplitude and frequency of spontaneous oscillations in EEG and depth electrograph were enhanced by chronically stimulating the dorsal HPC(n=12)and MTNC(n=11)respectively.Robust EEG rhythmic oscillations became prediction for behavioral and electrographic seizures in kindled rats.Tetanus-induced synchronous oscillations in the HPC-MTNC-cerebral cortex neural pathway could elicit the generation and development of epilepsy.
