The rh-CSF1 improves mitochondrial function and cell apoptosis in neurons under oxygen-glucose deprivation
10.3969/j.issn.1002-0152.2024.08.006
- VernacularTitle:rh-CSF1改善缺糖缺氧损伤神经元线粒体功能和细胞凋亡
- Author:
Rui LIU
1
;
Kuan FAN
;
Pengju ZHANG
;
Yu TIAN
;
Wei SI
;
Shirong LI
;
Lu WANG
;
Ran GU
;
Xiao HU
Author Information
1. 贵州省人民医院神经内科(贵阳 550000)
- Keywords:
Colony stimulating factor-1;
Oxygen-glucose deprivation;
Apoptosis;
Oxidative stress;
Mitochon-drial function;
Ischemic stroke
- From:
Chinese Journal of Nervous and Mental Diseases
2024;50(8):489-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which Colony Stimulating Factor-1(CSF1)inhibits apoptosis in neurons subjected to oxygen-glucose deprivation(OGD).Methods Primary rat cortical neurons were divided into the OGD damaged neuron model group(OGD group),the rh-CSF1 intervention group(rh-CSF1 group),and control group.The sample size for each group was 3.After intervention with recombinant human CSF1(rh-CSF1),neuronal apoptosis rate and intracellular ATP content,reactive oxygen species levels,mitochondrial membrane potential,and mitochondrial DNA copy number were measured.The content of malondialdehyde within mitochondria and the activity of superoxide dismutase were also assessed.Results Intervention with rh-CSF1 increased mitochondrial membrane potential(0.55±0.03 vs.0.43±0.06,P<0.01),mitochondrial DNA copy number(0.88±0.05 vs.0.72±0.06,P<0.05),ATP content[(15.70±0.99)mmol/mg vs.(11.70±1.00)mmol/mg,P<0.01)],and superoxide dismutase[(18.47±1.38)U/mg vs.(14.78±1.81)U/mg,P<0.05)]activity in neurons injured by OGD.It also reduced levels of rectivereactive oxygen species(3.64±0.21 vs.4.45±0.33,P<0.05)and malondialdehyde within mitochondria[(2.13±0.19)mmol/mg vs.(2.78±0.20)mmol/mg,P<0.05)],and inhibited neuronal apoptosis(10.12±0.78 vs.17.04±1.23,P<0.01)Conclusion rh-CSF1 may alleviate the damage in neurons induced by OGD by improving mitochondrial function,reducing oxidative stress,and inhibiting cell apoptosis.
