The electrophysiological characteristics of primary hamster cortical neurons with Mg2+-free external
10.3969/j.issn.1002-0152.2024.03.003
- VernacularTitle:无镁诱导仓鼠原代皮质神经元电生理学特性
- Author:
Qiuyue SHEN
1
;
Nana LIU
;
Lili LIU
;
Yuwu JIANG
;
Xinlin HOU
Author Information
1. 北京大学第一医院儿科(北京 100034)
- Keywords:
Epilepsy;
Syrian hamsters;
Cerebral cortex;
Cortical excitability;
Neuron;
Patch-clamp techniques;
Ex-citatory postsynaptic current;
Excitatory postsynaptic potential
- From:
Chinese Journal of Nervous and Mental Diseases
2024;50(3):140-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the electrophysiological characteristics of hamster primary cortical neurons by using the whole-cell patch-clamp recordings in a Mg2+-free cell model of epilepsy.Methods The cerebral cortex were isolated for primary neuron culture from neonatal Syrian hamsters 1-2 days after birth.After 12 days,primary neurons were exposed to normal external(Mg2+group)or Mg2+-free external(Mg2+-free group)respectively for 3 hours,followed by a 24-hour incubation in normal culture medium.Excitatory postsynaptic currents(EPSC)and excitatory postsynaptic potentials(EPSP)were recorded in voltage clamp mode and current clamp mode using whole-cell patch-clamp recordings,respectively.Results Compared to Mg2+group,the frequency of EPSC[(124.38±75.15)Hz vs.(33.93±22.32)Hz,P<0.001](P<0.001)and EPSP[(37.05±38.37)Hz vs.(5.63±9.52)Hz,P<0.01]in primary cortical neurons from the Mg2+-free group increased significantly.Meanwhile,there were no statistically significant differences in the amplitude,area under the curve and half-width of EPSC and EPSP between the two groups(P>0.05).Conclusion The present study demonstrates an increase in excitability of primary hamsters cortical neurons after Mg2+depletion,suggesting that these neurons can be utilized to use to create cell models of epilepsy.
