Molecular epidemiology of spotted fever group rickettsiae infections in wild rodents from Fengshan County,Guangxi
10.3969/j.issn.1002-2694.2024.00.151
- VernacularTitle:广西凤山县野鼠感染斑点热群立克次体的分子流行病学研究
- Author:
Si-Si CHEN
1
;
Fang-Ni WANG
;
Ze-Yun XU
;
Rui JIAN
;
Jing XUE
;
Wen-Ping GUO
Author Information
1. 承德医学院,基础医学院,承德 067000
- Keywords:
rodents;
SFGR;
Candidatus Rickettsia jingxinensis;
Rickettsia raoultii;
Rickettsia sibirica
- From:
Chinese Journal of Zoonoses
2024;40(10):989-993
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the prevalence of spotted fever group rickettsia(SFGR)in wild rodents collected from Fengshan County in the Guangxi Zhuang Autonomous Region,and to determine their species.Wild rodents were captured in cages in Fengshan County,Hechi City,Guangxi Zhuang Autonomous Region.The rodents were identified according to morphological characteristics,and the findings were confirmed through molecular biology methods.Subsequently,spleen samples were collected,and DNA was extracted.The outer membrane protein A(ompA)gene was amplified with semi-nested PCR to determine the species of SFGR in captured wild rodents.After sequencing of the PCR products,homology and phylogenetic analyses of ompA gene sequences were performed.A total of 105 wild rodents belonging to seven species were captured.FGR was detected in six rodent species(Bandicota indica,Leopoldamys edwardsi,Mus caroli,Mus Pahari,Rat-tus andamanensis,and Rattus losea,but not Berylmys bower si),and the total positivity rate was 23.8％.Three Rickettsia species,Candidatus Rickettsia jingxinensis,Rickettsia raoultii,and Rickettsia sibirica,were identified from analysis of the ompA gene sequence.This study revealed the presence of three species of SFGR infecting wild rodents from Fengshan County,Guangxi Zhuang Autonomous Region,thus suggesting that Fengshan County is a natural focus of tick-borne spotted fever.This study highlights the need to strengthen monitoring and prevention measures for rickettsiosis.
