Establishment of a PCR-RFLP typing method for Balantioides coli

VernacularTitle:结肠小袋纤毛虫PCR-RFLP分型方法的建立
Author: A-Hui XU ¹ ; Cai-Cai FENG ; Shan-Wang FENG ; Li-Zhuo ZHAO ; Wen-Xin QI ; Wen ZHANG ; Su-Hui HU ; Tian-Qi WANG ; Wen-Chao YAN
Author Information

1. 河南科技大学动物科技学院,洛阳 471023
Keywords: Balantioides coli; PCR-RFLP analysis; pig; sheep; guinea pig
From: Chinese Journal of Zoonoses 2024;40(9):829-833,840
CountryChina
Language:Chinese
Abstract: This study established an efficient and specific method for type analysis of genetic variants of Balantioides coli.The restriction endonucleases ApoI and PflMI were selected to digest the PCR amplification products of ITS1-5.8S rDNA-ITS2 of B.coli,and to establish a PCR-RFLP typing method.The PCR-RFLP method was subsequently used to analyze the genetic variants in clinical fecal samples from pigs,sheep,and guinea pigs.The PCR-RFLP method based on ApoI and PflMI accurately distinguished the main A and B genetic variants of B.coli,and further divided the main B type into B-c and B-t sub-types of genetic variants with PflMI.Compared with the results of microscopy and sequencing,the PCR-RFLP method showed good specificity and higher sensitivity,and was able to identify not only single but also multiple variants of B.coli in a single clinical sample.This study successfully established the PCR-RFLP method for B.coli,which can be used for genetic di-versity identification and molecular epidemiological studies of B.coli.