Drug resistance and phylo-typing of ESBL-producing Escherichia coli from diarrheic lambs in Kashgar area,Xinjiang
10.3969/j.issn.1002-2694.2024.00.114
- VernacularTitle:新疆喀什地区腹泻羔羊源产ESBL大肠杆菌的耐药性和系统进化分群研究
- Author:
Yun HU
1
,
2
;
Bai-Li ZHENG
;
Wei-Li CHEN
;
Ya-Ling CHENG
;
Lan MA
;
Pan-Pan TONG
;
Ying-Yu LIU
Author Information
1. 新疆农业大学动物医学学院,乌鲁木齐 830000
2. 新疆草食动物新药研究与创制重点实验室,乌鲁木齐 830000
- Keywords:
lamb with diarrhea;
Escherichia coli;
ESBL;
drug sensitivity test;
phylo-typing
- From:
Chinese Journal of Zoonoses
2024;40(8):716-722
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to determine the frequency and resistance patterns of ESBL-producing E.coli in lambs with diarrhea in the Kashi area,Xinjiang.The findings may provide guidance for the prevention and control of clinical E.coli disease.We collected 385 samples of perianal feces from lambs with diarrhea in the Kashgar area.From these samples,we isolated 371 strains of E.coli.We then used the double-paper-sheet synergistic method to screen for ESBL-producing E.coli.Additionally,we conducted analyses to identify drug-resistance genes,analyze drug resistance,and study the phylo-typing of the screened strains.Of 371 E.coli strains,204 were identified as ESBL-producing strains.The prevalence rates of blaCTX-M,blaCTX-M-1G,blaCTX-M-9G,and bla TEM resistance genes was 67.65％,69.12％,30.39％,and 63.73％,respectively.All ESBL-pro-ducing strains were resistant to multiple drugs,with resistance rates ranging from 90.69％to 100％for eight specific drugs:ampicillin,cefotaxime,gentamicin,enrofloxacin,azithromy-cin,tetracycline,chloramphenicol,methotrexate,and amitrazine.The phylogenetic subgroups of the strains were distributed primarily in groups A and D.Among group A strains,41.11％exhibited resistance to ten drugs,whereas among group D strains,40％exhibited resistance to 11 drugs.ESBL-pro-ducing strains of Escherichia coli are the main pathogens cau-sing diarrhea in lambs in the Kashgar region;group A is the main group,and all groups are multi-drug resistant.
