Genomic characteristics of the Vibrio cholerae O1 group isolated from humans in Fujian Province,2008 to 2022
10.3969/j.issn.1002-2694.2024.00.111
- VernacularTitle:2008-2022年福建省O1群霍乱弧菌人源株基因组特征研究
- Author:
Zi-Li KE
1
;
Xiao-Xuan ZHANG
;
Hai-Bin XU
;
Ya-Dong GAO
;
Chao-Chen LUO
;
Meng-Ying HUANG
;
Yu-Feng QIU
;
Jin-Song YANG
Author Information
1. 福建省疾病预防控制中心传染病预防控制所,福建省人兽共患病重点实验室,福州 350012
- Keywords:
Vibrio cholerae;
whole genome sequencing;
bioinformatics analysis;
core-genome multilocus sequence typing(cgMLST);
pan-genome
- From:
Chinese Journal of Zoonoses
2024;40(8):708-715
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at understanding the genomic characteristics of the Vibrio cholerae O1 group isolated from humans in Fujian Province,to provide essential data for the molecular epidemiological study of cholera.From 2008 to 2022,16 strains of the V.cholerae O1 group from patients and carriers were collected,and antibiotic sensitivity was determined accord-ing to the minimum inhibitory concentration(MIC).The whole genome sequences obtained through second generation sequen-cing were analyzed in open source software,including snippy,Roary,and Prokka,as well as online analysis websites,inclu-ding NCBI and BacWGSTdb,for core-genome multilocus sequence typing(cgMLST),core-genome single nucleotide polymor-phism analysis(cgSNP),virulence gene analysis,drug resistance gene prediction,and pan-genomic diversity analysis.The whole genome sequences of V.cholerae were divided into five sequence types(STs),among which the newly discovered ST182 and ST1480 were the evolutionary branches of the current dominant clonal group ST75 in China,and were highly related to two strains isolated from Taiwan in 2010 and 2013,respectively.Both toxigenic strains and non-toxigenic strains carried a variety of virulence factors and showed gene variation to varying degrees.Thirteen drug resistance genes in seven categories were predicted,among which the distribution of colistin and tetracycline resistance genes was consistent with the drug resistance phenotype.Pan-ge-nomic analysis indicated that V.cholerae had an open pan-genome,and Roary cluster analysis showed higher resolution than cgMLST.In summary,V.cholerae O1 group isolates from humans in Fujian Province have polymorphisms in genome structure and function,and the newly discovered ST1480 clone group has epidemic potential.Therefore,the monitoring of such strains must be strengthened.
