Antimicrobial resistance analysis and genomic characteristics of enteropathogenic Escherichia coli derived from ducks
10.3969/j.issn.1002-2694.2024.00.118
- VernacularTitle:鸭源肠致病性大肠杆菌的耐药特征及全基因组学分析
- Author:
Jun-Lin LI
1
;
Jia-Meng HU
;
Luo WANG
;
Jia-Rui LI
;
Hao-Tian LIU
;
Jing XIA
;
Min CUI
;
Li-Kou ZOU
;
Xin-Feng HAN
Author Information
1. 四川农业大学动物医学院,成都 611130
- Keywords:
enteropathogenic Escherichia coli;
antimicrobial resistance;
whole-genome sequencing;
antimicrobial resistance gene;
virulence gene
- From:
Chinese Journal of Zoonoses
2024;40(8):701-707
- CountryChina
- Language:Chinese
-
Abstract:
Enteropathogenic Escherichia coli(EPEC),a zoonotic foodborne pathogen,can induce severe and prolonged di-arrhea,thus substantially affecting global public health safety.To understand the pathogenicity of EPEC and its potential risk to human health,this study investigated the antimicrobial resistance and genome-wide characteristics of EPEC originating from ducks.After identification of EPEC with the plate method and PCR,antimicrobial susceptibility of the isolates was examined with the microbroth dilution method.In addition,analyses of serotype,sequence type(ST),and plasmid incompatibility groups were conducted with whole-genome sequencing(WGS)and bioinformatic methods.Ten EPEC isolates were identified,including serotypes O71∶H40 and O3∶H21.All EPEC strains exhibited multiple drug resistance.The highest proportion of resistance(100％)was observed to ciprofloxacin,streptomycin,tetracycline,and polymyxin B.In contrast,the isolates showed susceptibility to cefoxitin,amikacin,and imipenem.Furthermore,all strains carried the tetracycline resistance gene tet(A)and extended-spectrum β-lactamase(ESBL)resistance genes,including blaOXA-10,blaTEM-1A,and blaTEM-1B.Various virulence genes,associated primarily with the secretory system,were de-tected in the isolates.However,no bf p genes or per ARC genes were identified,thus indicating that the EPEC isolates were atypical EPEC(aEPEC).The results demonstrated the presence of multiple antimicrobial resistance,multiple resistance and viru-lence genes,and various plasmid incompatibility groups,thus in-dicating potential pathogenicity to humans.Strengthened monitoring of duck-derived EPEC is crucial to effectively control the spread of the pathogen and safeguard public health.
