Drug sensitivity and genomic characteristics of a strain of Listeria monocytogenes ST5 isolated from a neonate
10.3969/j.issn.1002-2694.2024.00.103
- VernacularTitle:从新生儿分离到1株ST5型单增李斯特菌的药敏及基因组特征分析
- Author:
Zeng-Bin LIU
1
;
Li LIU
;
Zhi-Rong LI
;
Cai-Hong XU
;
Hong-Bin WANG
;
Ru-Gang YANG
;
Tao FAN
;
Jian-Hong ZHAO
;
Jing-Rui ZHANG
Author Information
1. 河北医科大学第二医院,石家庄 050000
- Keywords:
neonatal;
Listeria monocytogenes;
phenotypic drug sensitivity;
whole genome sequencing
- From:
Chinese Journal of Zoonoses
2024;40(7):644-651
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to determine the drug resistance phenotype and genetic characteristics of Listeria monocytogenes ST5 LK100 isolated from a neonate,which provided a basis for the diagnosis and treatment of L.monocyto-genes infection and to enhance the understanding of the genomic characteristics of this strain.A suspected L.monocytogenes strain was isolated from the gastric juice sample of an infected neonate,and identified with a VITEK2 Compact automatic mi-crobial identification instrument and 16S RNA sequencing.Five drug sensitivity tests were conducted on the identified strain with the E-test method.Additionally,the whole genome of the strain was sequenced using a third-generation sequencing plat-form.The antibiotic resistance elements of the strain were identified by BlastN with the CARD antibiotic resistance gene data-base.The multilocus sequence typing(MLST),serotyping,and virulence genes of the strain was determined by Pasteur da-tabase,the virulence gene distribution was analyzed using the virulence analysis website.The prophages of the strain were predicted and annotate by PHASTER online website.The strain(LK100)isolated from the neonate was identified as L.monocytogenes.This strain was sensitive to penicillin,ampicil-lin,meropenem,erythromycin,and trimethoprim-sulfame-thoxazole antibiotics.The MLST type and serotype was ST5 and 1/2b-3b,respectively.The total length of the chromoso-mal genome of LK100 was 3 032 582 bp with a GC content of 37.91％,and it contained a complete circular plasmid with a se-quence length of 52 822 bp.The strain LK100 carried complete InlA protein,LIPI-1 pathogenicity island,SSI-1 stress survival island,and an LGI2 genomic island.The intrinsic antibiotic resistance genes were mainly located on the chromosome.Five prophage sequences were predicted in the LK100 genome.This study identified a strain of ST5 L.monocytogenes LK100 from an infected neonate and characterized its genome and antibiotic sensitivity,laying the foundation for further research on ST5 L.monocytogenes.
