Effect of LAG3 molecule on B lymphocyte subsets and its function in the liver of mice infected with Echinococcus multilocularis
10.3969/j.issn.1002-2694.2024.00.091
- VernacularTitle:LAG3分子对泡球蚴感染小鼠肝脏B淋巴细胞亚群及其功能的影响
- Author:
Xu-Ran ZHENG
1
,
2
;
Bing-Qing DENG
;
Xue-Jiao KANG
;
Yin-Shi LI
;
Ainiwaer ABIDAN
;
Qian YU
;
Rousu ZIBIGU
;
Duolikun ADILAI
;
Mao-Lin WANG
;
Hui WANG
;
Chuan-Shan ZHANG
;
Jing LI
Author Information
1. 新疆医科大学基础医学院,乌鲁木齐 830054
2. 新疆医科大学第一附属医院临床医学研究院、省部共建中亚高发病成因与防治国家重点实验室、新疆维吾尔自治区包虫病基础医学重点实验室,乌鲁木齐 830054
- Keywords:
Echinococcus multilocularis;
LAG3;
B cells;
B cell subsets
- From:
Chinese Journal of Zoonoses
2024;40(6):529-536
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at investigating the effect of lymphocyte activation gene-3(LAG3)on liver B lymphocyte subsets and their functions in WT and LAG3-KO mice infected with Echinococcus multilocularis(E.multilocularis).In a mouse model of E.multilocularis infection,the expression and localization of CD19 and α-SMA in liver were detected by immu nohistochemistry.CD80,CD86 and MHC-Ⅱ molecules expressed on B cells and their subsets in mice liver were detected by flow cytometry.After 12 weeks of infection,the area and percentage of CD19 in LAG3-KO group was slightly higher than that in WT group,but the difference was not statistically(t=-1.241、-1.237,P＞0.05).The area and percentage of a-SMA in LAG3-KO group was higher than that in WT group(t=-3.224、-3.227,P＜0.05).The proportion of CD80 and MHC-Ⅱ molecules expressed on liver B cells in LAG3-KO group was up-regulated(t=-2.379,-3.321,P＜0.05).The percentage of liver B2 cells in LAG3-KO group was higher than that in WT group(t=-2.695,P＜0.05).The expression of CD80 on Blb cells in LAG3-KO group was significantly up-regulated(t=-5.315,P＜0.001).The proportion of CD80 of B2 cells in LAG3-KO group was lower than that in WT group(t=2.806,P＜0.05).The expression of MHC-Ⅱ molecule in B2 cells in LAG3-KO group was up-regulated(t=-4.227,P＜0.01).It is suggested that LAG3 molecules affected the B cell subsets and func-tion of mouse liver in the middle stage of E.multilocularis infection,especially B2 lymphocytes.LAG3 molecule exerted an in-hibitory effect on the activation of B cells and the expression of MHC-class Ⅱ molecules,suggesting that it may be involved in B cell exhaustion caused by E.multilocularis.
