Genomic and cellular infection characteristics of a newly isolated Mangshi virus in China
10.3969/j.issn.1002-2694.2024.00.087
- VernacularTitle:我国新分离芒市病毒基因组特征与细胞感染特性研究
- Author:
Heng YANG
1
,
2
;
Zhan-Hong LI
;
Lei XIAO
;
Zhuo-Ran LI
;
Jia-Rui XIE
;
De-Fang LIAO
;
Lin GAO
;
Hua-Chun LI
Author Information
1. 昆明学院,农学与生命科学学院,昆明 650214
2. 云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,昆明 650224
- Keywords:
Mangshi virus;
complete genome sequencing;
reassortment;
celltropism;
epidemiological survey
- From:
Chinese Journal of Zoonoses
2024;40(6):504-511,528
- CountryChina
- Language:Chinese
-
Abstract:
The genomic characteristics and cellular tropism of a Mangshi virus(MSV)isolated in China were investigated,thereby establishing a robust foundation for further research on the evolution and pathogenicity of MSV.The genome sequence of MSV strain V301/YNJH/2019 was obtained by next-generation sequencing,followed by phylogenetic tree construction and rearrangement assessment using software IQtree,RPD4,and Simplot.Viral proliferation was assessed in C6/36(Aedes albop-ictus),Vero(African green monkey kidney),and BHK(baby hamster kidney)cells.An initial epidemiological investigation of MSV in local cattle and goats was conducted using the serum neutralization test.The genome of MSV strain V301/YNJ H/2019 was 20623 bp in length,encompassing 12 segments of double-stranded RNA(Seg-1 to Seg-12).Sequence analysis confirmed genomic rearrangement of the Seg-1 and Seg-11 sequences,ex-hibiting high similarity to MSV isolated from lake sediment in China in 2022,while Seg-2 to Seg-10 and Seg-12 were most closely related to a MSV strain isolated from mosquitoes in China in 2013.The virus efficiently proliferated and induced sig-nificant cytopathic effects(CPE)in both C6/36 and BHK cells,but limited replication and no observable CPE in Vero cells.No detectable neutralizing antibodies against MSV were detected in 20 goat serum samples collected in Mangshi,while 2 of 20 bo-vine serum samples were positive with neutralizing antibody titers of 1:128 and 1:54.Whole genome sequencing revealed re-assortment events of the V301/YNJH/2019 strain,which is capable of infecting C6/36,BHK,and Vero cells.MSV infection was confirmed in cattle in Mangshi.
