Expression and activity analysis of Clostridium difficile toxin B type 2
10.3969/j.issn.1002-2694.2024.00.076
- VernacularTitle:艰难梭菌毒素B2型蛋白重组表达及活性分析
- Author:
Xing-Hao LIN
1
;
Kai ZHANG
;
Meng-Jie WANG
;
Ming YANG
;
Han-Yang GU
;
Xiao-Lan XUE
;
Yong-Neng LUO
;
Da-Zhi JIN
;
Hui HU
Author Information
1. 杭州医学院检验医学院、生物工程学院,杭州 310053
- Keywords:
Clostridium difficile;
toxin B;
Bacillus subtilis;
expression;
cytotoxicity
- From:
Chinese Journal of Zoonoses
2024;40(6):498-503
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at creating an engineered strain of Bacillus subtilis for efficient expression of biologically active type 2 toxin B(TcdB2)derived from a highly virulent strain of Clostridium difficile.The TcdB2 gene was cloned from ST1/RT027 strain genome DNA,incorporated into the PHT01 vector,and then transformed into B.subtilis strain WB800N for prokaryotic expression.Cell toxicity assays revealed that the recombinant TcdB2 exhibited cytotoxic effects in various cells.The engineered B.subtilis strain effectively expressed biologically active TcdB2,thus providing a basis for further exploration of the pathogenic mechanisms of highly virulent strains of C.difficile and establishing a foundation for potential vaccine can-didate targets.
