Genetic diversity of GⅡ genogroup noroviruses linked to clustered infections in Northeast Chongqing,2021-2022
10.3969/j.issn.1002-2694.2024.00.067
- VernacularTitle:渝东北片区聚集性诺如病毒感染毒株的遗传多样性
- Author:
Zhong-Kai LANG
1
;
Ai-Ping CHEN
;
Heng-Qin WANG
;
Yu-Lu GAN
;
Yong-Jun ZHANG
Author Information
1. 重庆市万州区疾病预防控制中心,重庆 404100
- Keywords:
norovirus;
genotyping;
phylogenetic analysis
- From:
Chinese Journal of Zoonoses
2024;40(5):448-453
- CountryChina
- Language:Chinese
-
Abstract:
Norovirus is the global leading cause of epidemic and endemic acute gastroenteritis in people of all ages.To inves-tigate the genetic diversity of GⅡ genogroup noroviruses linked to clustered infections in northeast Chongqing,we collected anal swabs or environmental smears from 11 norovirus outbreaks during 2021-2022.Norovirus RNA was detected by quantitative real-time PCR(qRT-PCR),and partial viral RdRp/capsid genes were amplified by reverse transcription PCR(RT-PCR)and sequenced.Among samples from 11 outbreaks in 4 districts and counties,55 strains of GⅡ genogroup norovirus were detected.Six genotypes were identified with an online norovirus genotyping tool(http://www.rivm.nl/mpf/norovirus/typingtool).Genotype GⅡ.17[P17]was associated with four outbreaks;the co-circulating GⅡ.17[P17]and GⅡ.1[P16]caused another out-break;GⅡ.6[P7]and GⅡ.8[P8]respectively were linked to two outbreaks;and GⅡ.3[P12]and GⅡ.2[P16]respectively ac-counted for one outbreak.Phylogenetic analysis also indicated that 55 GⅡ genogroup strains formed five clusters,with norovir-uses of identical genotypes from diverse events belonging to the same cluster,and that genetically distinct genotypes from di-verse events belonged to different clusters.Therefore,our results revealed that multiple genotypes associated with norovirus outbreaks were circulating in northeast Chongqing,and GⅡ.17[P17]was the predominant genotype linked to these out-breaks during 2021-2022.Most norovirus outbreak events were caused by single sources,and genetic relationships were demonstrated among noroviruses of identical genotypes from diverse events.
