Porcine SIRT5 promotes replication of foot and mouth disease virus type O in PK-15 cells
10.3969/j.issn.1002-2694.2024.00.063
- VernacularTitle:猪源SIRT5促进O型口蹄疫病毒在PK-15细胞复制
- Author:
Guo-Hui CHEN
1
,
2
;
Xi-Juan SHI
;
Xin-Tian BIE
;
Xing YANG
;
Si-Yue ZHAO
;
Da-Jun ZHANG
;
Deng-Shuai ZHAO
;
Wen-Qian YAN
;
Ling-Ling CHEN
;
Mei-Yu ZHAO
;
Lu HE
;
Hai-Xue ZHENG
;
Xia LIU
;
Ke-Shan ZHANG
Author Information
1. 甘肃农业大学生命科学技术学院,兰州 730070
2. 中国农业科学院兰州兽医研究所,兰州大学动物医学与生物安全学院动物疫病防控国家重点实验室,兰州 730046
- Keywords:
porcine SIRT5;
FMDV-O;
interferon stimulating gene;
PK-15 cells
- From:
Chinese Journal of Zoonoses
2024;40(5):421-429
- CountryChina
- Language:Chinese
-
Abstract:
The effect of porcine SIRT5 on replication of foot and mouth disease virus type O(FMDV-O)and the underlying regulatory mechanism were investigated.Western blot and RT-qPCR analyses were employed to monitor expression of endoge-nous SIRT5 in PK-15 cells infected with FMDV-O.Three pairs of SIRT5-specific siRNAs were synthesized.Changes to SIRT5 and FMDV-O protein and transcript levels,in addition to virus copy numbers,were measured by western blot and RT-qPCR analyses.PK-15 cells were transfected with a eukaryotic SIRT5 expression plasmid.Western blot and RT-qPCR analyses were used to explore the impact of SIRT5 overexpression on FMDV-O replication.Meanwhile,RT-qPCR analysis was used to detect the effect of SIRT5 overexpression on the mRNA expression levels of type I interferon-stimulated genes induced by SeV and FMDV-O.The results showed that expression of SIRT5 was up-regulated in PK-15 cells infected with FMDV-O and siRNA interfered with SIRT5 to inhibit FMDV-O replication.SIRT5 overexpression promoted FMDV-O replication.SIRT5 over-expression decreased mRNA expression levels of interferon-stimulated genes induced by SeV and FMDV-O.These results suggest that FMDV-O infection stimulated expression of SIRT5 in PK-15 cells,while SIRT5 promoted FMDV-O rep-lication by inhibiting production of type I interferon-stimula-ted genes.These findings provide a reference to further ex-plore the mechanism underlying the ability of porcine SIRT5 to promote FMDV-O replication.
