Genetic diversity of Ixodes persulcatus in parts of Inner Mongolia
10.3969/j.issn.1002-2694.2024.00.056
- VernacularTitle:内蒙古部分地区全沟硬蜱的遗传多样性研究
- Author:
Meng-Yu CUI
1
;
Si SU
;
Li-Li XING
;
Lan MU
;
Rui-Juan GAO
;
Qi-Qi GUO
;
Hong REN
;
Dong-Dong QI
;
Jing-Feng YU
Author Information
1. 内蒙古医科大学公共卫生学院,呼和浩特 010100
- Keywords:
Ixodes persulcatus;
16S rRNA;
COI gene;
genetic diversity
- From:
Chinese Journal of Zoonoses
2024;40(4):295-301
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to clarify the genetic diversity and population history of Ixodes persulcatus in some ar-eas of Inner Mongolia in order to provide accurate data for effective vector control programs and reveal the transmission mecha-nism.Samples were collected in 10 areas of Inner Mongolia during the active tick season(April 2021-July 2023)using the flag-dragging and manual sampling methods.The 16S rRNA and COI gene were sequenced to clarify genetic diversity of I.per-sulcatus.The positivity rates for the COI gene and 16S rRNA were 90.00％and 98.33％respectively.Overall,18 and 15 haplotypes were identified for the COI gene and 16S rRNA,respectively,with a total haplotype diversity＞0.762 and total nucleotide diversity＜0.005.The Tajima's values and Fu's Fs were negative for significance.A nucleotide mismatch map was shown as a single peak.The genetic differentiation index FST of the population indicates a small degree of genetic differ-entiation of the population,while analysis of molecular vari-ance indicates that the variation within populations was greater than between populations.Phylogenetic tree and haplotype network plots showed confounding distributions between hap-lotypes.I.persulcatus from the Hinggan League and Hulun-buir regions can adapt to environmental changes and possess abundant genetic diversity.Genetic differentiation is mainly concentrated within the population and no geographical isolation was observed.
