Morphological classification and molecular identification of Hyalomma asiaticum in parts of Xindi Township,Xinjiang
10.3969/j.issn.1002-2694.2024.00.050
- VernacularTitle:新疆新地乡部分地区亚洲璃眼蜱的形态及分子生物学鉴定
- Author:
Xiao-Qing ZAN
1
,
2
;
Qiao-Yun REN
;
Jin LUO
;
Yan-Long WANG
;
Pei-Wen DIAO
;
Li-Yan CHE
;
Jian-Xun LUO
;
Hong YIN
;
Gui-Quan GUAN
;
Guang-Yuan LIU
;
Hong-Xi ZHAO
Author Information
1. 宁夏大学动物科技学院,银川 750021
2. 中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃省动物寄生虫病重点实验室,兰州 730046
- Keywords:
Hyalomma asiaticum;
morphological identification;
COI gene;
ITS2 gene
- From:
Chinese Journal of Zoonoses
2024;40(4):289-294
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to identify the tick species native to Xindi Township,Yumin County,Xinjiang,China.Preliminary morphological identification of parasitic ticks collected from animals in the area was conducted with an ultra-depth of field three-dimensional VHX 600 digital stereo microscope.Total DNA of the ticks was extracted,amplified by PCR based on the COI and ITS2 gene loci,and the posi-tive PCR products were sequenced.The sequence were a-ligned with reference sequences from the NCBI database were aligned with the Basic Local Alignment Search Tool.A genet-ic phylogenetic tree was generated with the neighbor-joining method of MEGA 7.0 software to determine the evolutionary biological characteristics of ticks.Morphological identification showed that the ticks collected from Xindi Township of Yu-min County were consistent with the characteristics of Hya-lomma asiaticum.An evolutionary tree based on the COI and ITS2 gene sequences showed that the ticks collected in this study were clustered with known H.asiaticum sequences.The PCR products of COI and ITS2 were sequenced and compared,which confirmed that the collected tick species were H.asiaticum,in agreement with the morphological and molecular biological results.These findings help to clarify the distribution of ticks in Xindi Township of Xinjiang,and provide basic data for the analysis of tick genetic and evolutionary characteristics,as reference for surveillance and control of ticks in the Xinjiang Uygur Autonomous Region.
