The targeting relationship and function of miR-199b-3p and CRIM1 in gastric cancer cells
10.7659/j.issn.1005-6947.2024.10.014
- VernacularTitle:miR-199b-3p与CRIM1在胃癌细胞中的靶向关系及其功能
- Author:
Qinxi WANG
1
;
Jiong ZHANG
;
Kangming CHE
Author Information
1. 甘肃省天水市第一人民医院肿瘤外科,甘肃天水 741000
- Keywords:
Stomach Neoplasms;
MicroRNAs;
CRIM1;
Cell Proliferation;
Neoplasm Invasiveness
- From:
Chinese Journal of General Surgery
2024;33(10):1679-1687
- CountryChina
- Language:Chinese
-
Abstract:
Background and Aims:Studies have shown that miR-199b-3p is downregulated in gastric cancer tissues,while cysteine-rich transmembrane BMP regulator 1(CRIM1)is upregulated in these tissues.However,the role and mechanism of miR-199b-3p in the biological behavior of gastric cancer cells are still unclear,as is its potential association with CRIM1.Therefore,this study was conducted to investigate whether there is an interaction between miR-199b-3p and CRIM1 and how they affect the function of gastric cancer cells. Methods:qRT-PCR and immunohistochemistry were used to detect the expression levels of miR-199b-3p and CRIM1 in gastric cancer tissues and adjacent non-cancerous tissues.Gastric cancer MGC803 cells were used to assess changes in cell proliferation,invasion/migration abilities,and apoptosis rates after overexpression of miR-199b-3p(using miR-199b-3p mimics)or knockdown of CRIM1(using si-CRIM1).Bioinformatics analysis was used to predict the targeting relationship between miR-199b-3p and CRIM1,which was further validated by dual-luciferase reporter assay and confirmed through Western blot analysis. Results:The results of qRT-PCR indicated that,compared to adjacent non-cancerous tissues,miR-199b-3p expression was significantly lower in gastric cancer tissues,while CRIM1 expression was higher(both P＜0.05).Immunohistochemistry results demonstrated positive expression of CRIM1 in cancerous tissues,while it was negative in non-cancerous tissues.Overexpression of miR-199b-3p or CRIM1 knockdown resulted in decreased proliferation and invasion/migration abilities of MGC803 cells,along with increased apoptosis rates(all P＜0.05).Bioinformatics prediction and dual-luciferase reporter assays confirmed that CRIM1 is a target of miR-199b-3p.Western blot analysis showed that CRIM1 expression was significantly reduced after transfection with miR-199b-3p mimics(P＜0.05). Conclusion:CRIM1 is a target gene of miR-199b-3p,which can inhibit the proliferation,invasion,and migration of gastric cancer cells while promoting apoptosis by targeting and regulating CRIM1 activity.The miR-199b-3p/CRIM1 pathway may serve as a potential therapeutic target for gastric cancer.
