Clinical application of serum microRNA-198 and microRNA-365 combined with gastroscopy in diagnosis of chronic atrophic gastritis
- VernacularTitle:血清微RNA-198和微RNA-365联合胃镜检查在慢性萎缩性胃炎诊断中的应用效果
- Author:
Guangshu LI
1
;
Fen HU
;
Bibo ZHONG
Author Information
- Keywords: chronic atrophic gastritis(CAG); miRNA-198; miRNA-365; gastroscopy; diagnosis
- From: China Journal of Endoscopy 2024;30(6):23-29
- CountryChina
- Language:Chinese
- Abstract: Objective To analyze the value of serum microRNA-198(miRNA-198)and miRNA-365 combined with gastroscopy in diagnosis of chronic atrophic gastritis(CAG).Methods 180 patients with CAG who were treated from January 2022 to January 2023 were regarded as the observation group,and 180 patients with chronic non-atrophic gastritis in the same period were studied as control group.According to the degree of atrophy,patients with CAG were grouped into 56 mild cases,84 moderate cases and 40 severe cases.The subjects were examined by gastroscopy,and the relative expression levels of miRNA-198 and miRNA-365 in serum were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).The diagnostic value of serum miRNA-198 and miRNA-365 levels in patients with CAG was analyzed by receiver operator characteristic curve(ROC curve).The diagnostic value of serum miRNA-198 and miRNA-365 combined with gastroscopy for CAG was analyzed with four grid tables.Results Compared with the control group,the levels of serum miRNA-198 and miRNA-365 in patients with CAG in the observation group were obviously lower,with the aggravation of atrophy,the levels of serum miRNA-198 and miRNA-365 decreased in turn,the differences were statistically significant(P<0.05);the area under the curve(AUC)of serum miRNA-198 and miRNA-365 levels in the diagnosis of CAG was 0.898 and 0.945,respectively,the sensitivity,specificity and accuracy of combined gastroscopy in the diagnosis of CAG were 98.33%,81.11%and 89.72%,respectively,its diagnostic efficacy was higher than that of serum miRNA-198,miRNA-365 and gastroscopy alone.Conclusion The levels of serum miRNA-198 and miRNA-365 in patients with CAG are decreased,they have certain diagnostic value for CAG,their combination with gastroscopy can obviously improve the diagnostic value of CAG.
