miR-515-5p inhibits proliferation,migration and invasion of esophageal cancer cells by targeting TPX2-IL-6 axis
10.3969/j.issn.1000-484X.2024.12.017
- VernacularTitle:miR-515-5p通过靶向TPX2-IL-6轴抑制食管癌细胞增殖、迁移和侵袭
- Author:
Zhigao YUAN
1
;
Mingcai SHUI
;
Hui ZHOU
Author Information
1. 重庆市开州区人民医院肿瘤科,重庆 405400
- Keywords:
miR-515-5p;
TPX2;
IL-6;
Esophageal cancer;
Proliferation;
Migration;
Invasion
- From:
Chinese Journal of Immunology
2024;40(12):2559-2564
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore inhibiting effect of miR-515-5p on proliferation,migration and invasion of esophageal cancer cells by targeting TPX2-IL-6 axis.Methods:Esophageal cancer cells EC9706 were treated with IL-6,and divided into Con group,IL-6 group,IL-6+miR-NC group,IL-6+miR-515-5p group,IL-6+miR-515-5p+pcDNA group,IL-6+miR-515-5p+TPX2 group.qRT-PCR was used to detect expressions of miR-515-5p and TPX2 mRNA;clone formation experiment and Transwell were used to de-tect cell proliferation,migration and invasion;Western blot was used to detect CyclinD1,MMP2,MMP9 and TPX2 protein expres-sions;dual luciferase report experiment was used to detect targeting relationship between miR-515-5p and TPX2.Results:Compared with Con group,expression of miR-515-5p in IL-6 group was decreased,expressions of TPX2 mRNA and protein were increased,numbers of cloned,migrating and invasion cells were increased,protein expressions of CyclinD1,MMP2 and MMP9 were increased.Compared with IL-6+miR-NC group,expression of miR-515-5p in IL-6+miR-515-5p group was increased,expressions of TPX2 mRNA and protein were decreased,numbers of cloned,migrating and invasion cells were reduced,CyclinD1,MMP2,MMP9 protein expressions were decreased.miR-515-5p targeting regulated expression of TPX2.Overexpression of TPX2 could reverse effect of up-regulation of miR-515-5p on IL-6-induced proliferation,migration and invasion of esophageal cancer cells.Conclusion:miR-515-5p can targeting inhibit TPX2 to inhibit IL-6-induced proliferation,migration and invasion of esophageal cancer cells.
