Vitexin exerts anti-prostate cancer effects by modulating macrophage polari-zation from M2 to M1
10.3969/j.issn.1000-484X.2024.12.016
- VernacularTitle:牡荆素通过调控巨噬细胞M2向M1极化发挥抗前列腺癌作用的机制
- Author:
Shijia LIANG
1
;
Jianming SUN
;
Wenjun HAN
;
Yiqun SHAO
;
Peng LIU
;
Junbo WANG
;
Bowen LIANG
;
Jianmin MAO
Author Information
1. 上海中医药大学附属第七人民医院,上海 200137
- Keywords:
Prostate cancer;
Macrophage;
Polarization;
Vitexin
- From:
Chinese Journal of Immunology
2024;40(12):2554-2558,2564
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of vitexin on macrophage polarization and its impact on tumor growth in a mouse model of prostate cancer.Methods:C57BL/6J male mice were used to establish RM-1 prostate cancer xenograft model.Mice were ran-domly divided into model group,vitexin-low,medium and high doses groups(40,80,160 mg/kg),and cisplatin group as positive control.After continuous administration for 16 days,mice were euthanized and tumor mass was measured.HE staining was performed to observe tumor morphology.Immunohistochemistry was used to detect Ki67 positive rate.Flow cytometry was conducted to measure expressions of CD86+CD11b and CD206+CD11b in tumor-associated macrophages.CCK8 assay was performed to assess cytotoxic effect of vitexin on RAW264.7 macrophages to determine suitable concentrations.RT-qPCR was used to measure mRNA expressions of M2 macrophage markers,including arginase-1(ARG-1),Fizz1 and Ym1.Results:Vitexin inhibited tumor volume and weight,induced tumor tissue necrosis,suppressed Ki67 protein expression,increased expression of CD86+CD11b+M1 macrophages,and inhibited CD206+CD11b+M2 macrophage expression in mouse tumor tissues in vivo.Vitexin at concentrations of 10～20 μmol/L showed no cyto-toxicity on RAW264.7 macrophages in vitro,and promoted expression of iNOS in IL-4-induced M2 macrophages while inhibiting CD206 expression,as well as suppressed mRNA expressions of ARG-1,Fizz1 and Ym1.Conclusion:Vitexin effectively inhibits tumor growth in a mouse model of prostate cancer,possibly by regulating M2 macrophages towards an M1 phenotype and exerting immunomodulatory effects.
