Effect of piperine on AC/PKA/CREB signaling pathway on hippocampal neuron apoptosis in rats with cerebral infarction
10.3969/j.issn.1000-484X.2024.12.013
- VernacularTitle:胡椒碱调节AC/PKA/CREB信号通路对脑梗死大鼠海马神经元凋亡的影响
- Author:
Deyan LI
1
;
Zongwen HUANG
;
Yadan WU
;
Xiuhong CHEN
Author Information
1. 海南省老年病医院中医科,海口 571100
- Keywords:
Piperine;
Cerebral infarction;
AC/PKA/CREB signaling pathway;
Neuron
- From:
Chinese Journal of Immunology
2024;40(12):2537-2542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and of piperine(PIP)on nerve injury in rats with cerebral infarction.Meth-ods:The rat model of cerebral infarction was prepared by modified thread embolism method.The rats were divided into Sham group,Model group,piperine group(PIP group,20 mg/kg piperine),piperine+PKA inhibitor group(PIP+H-89 group,20 mg/kg PIP+5 mg/kg H-89).The rats were scored for neurological impairment,the volume of cerebral infarction,neuronal damage and neuronal apoptosis were observed,and the levels of cAMP,IL-1β and IL-6 in hippocampus and the protein expressions of GFAP,NSE,AC6,PKA,p-CREB,CREB and BDNF were detected.Results:Compared with Sham group,Model group had large cerebral infarction,cerebral in-farction volume,the neurological deficit score,neuronal apoptosis rate,the levels of IL-6 and IL-1β in hippocampus,and number of GFAP positive cells,the positive expression rates of NSE protein increased,the number of Nissl bodies,and the expressions of cAMP,AC6,PKA,p-CREB and BDNF proteins in hippocampus decreased(P<0.05);compared with Model group,cerebral infarc-tion volume,the neurological deficit score,neuronal apoptosis rate,the levels of IL-6 and IL-1β in hippocampus,and number of GFAP positive cells,the positive expression rates of NSE protein in the PIP group decreased,the number of Nissl bodies,and the ex-pressions of cAMP,AC6,PKA,p-CREB and BDNF proteins in hippocampus increased(P<0.05);the effect of piperine in improving nerve injury in rats with cerebral infarction was able to be reversed by H-89.Conclusion:PIP can inhibit the apoptosis of hippocampal neurons,and improve the neural injury induced by cerebral infarction by activating AC/PKA/CREB signal pathway.
