Effects of vitamin B1 on function of splenic lymphocytes of mice in simulated microgravity
10.3969/j.issn.1000-484X.2024.12.006
- VernacularTitle:模拟微重力环境维生素B1对小鼠脾淋巴细胞功能的影响
- Author:
Shaoyan SI
1
;
Yingying WU
;
Yaya QIN
;
Ying SHANG
;
Xiaoyu MA
;
Shujun SONG
Author Information
1. 战略支援部队特色医学中心综合基础研究室,北京 100101
- Keywords:
Vitamin B1;
Simulated microgravity;
Lymphocytes;
Cell proliferation;
Cytokines
- From:
Chinese Journal of Immunology
2024;40(12):2496-2499,2505
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the effect of vitamin B1 on lymphocyte function in simulated microgravity.Methods:Splenocytes of mice were isolated,and the rotatary cell culture system was used to simulate microgravity.Lymphocytes were stimulated with mitotic agents Concanavalin A,and cells were treated with different concentrations of vitamin B1,proliferation indexes of lympho-cytes and levels of cytokines in supernatant were detected.Results:Simulated microgravity could inhibit proliferation of splenic lym-phocytes,and decrease levels of cytokines,while vitamin B1 could promote lymphocyte proliferation and cytokines production in cells cultured in simulated microgravity in a dose dependent manner.Conclusion:Vitamin B1 may attenuate the inhibitory effect of simulated microgravity on lymphocytes by regulating cell proliferation and secretion of cytokines.
