Current situation and application prospect of human immunodeficiency virus reservoir detection methods
10.3969/j.issn.1000-484X.2024.11.035
- VernacularTitle:人类免疫缺陷病毒储存库检测方法的现状及应用前景
- Author:
Haomei ZHAO
1
;
Qingyan ZHANG
;
Feng SANG
;
Yaoyao YANG
;
Bowen DENG
;
Shuoyang YUAN
;
Qiang LI
Author Information
1. 河南中医药大学药学院,郑州 450000
- Keywords:
Human immunodeficiency virus;
Reservoir;
Virus growth assay;
PCR;
Detection method
- From:
Chinese Journal of Immunology
2024;40(11):2444-2451
- CountryChina
- Language:Chinese
-
Abstract:
Acquired immune deficiency syndrome is an infectious disease with high mortality caused by human immunodefi-ciency virus(HIV).Even after antiretroviral therapy,the virus reservoir formed by the provirus integrated in the host genome can evade host immune surveillance and clearance.The existing methods of HIV reservoir detection mainly include cell culture induced virus growth assay in vitro and direct detection of the provirus genome based on PCR technology.Understanding and measuring accurately of HIV reservoir can provide reliable technical basis for HIV treatment research.This article summarizes and analyzes various detection methods of HIV reservoirs in recent years and their advantages and disadvantages,in order to select the suitable method for different detection objects of HIV reservior,and give appropriate suggestions,to provide technical support and theoretical guidance for further research on acquired immune deficiency syndrome.
