Impact of celastrol on angiogenesis in rats with endometriosis by regulating Hippo-YAP signal pathway
10.3969/j.issn.1000-484X.2024.11.015
- VernacularTitle:南蛇藤素调节Hippo-YAP信号通路对子宫内膜异位症大鼠血管生成的影响
- Author:
Jing ZHANG
1
;
Dongdong GAO
;
Cancan GU
;
Jing CHEN
Author Information
1. 黄淮学院医学院,驻马店 463000
- Keywords:
Celastrol;
Hippo-YAP signal pathway;
Endometriosis;
Angiogenesis
- From:
Chinese Journal of Immunology
2024;40(11):2330-2334,2342
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the impact of celastrol(CEL)on angiogenesis in rats with endometriosis(EM)and its regu-latory mechanism on Hippo-YAP signal pathway.Methods:SD female rats were grouped into six groups:sham operation group,model group,positive drug group(gestrinone),Verteporfin group(YAP inhibitor),low and high doses CEL groups,with 12 rats in each group.The weight and volume of rat endometrium were measured;HE staining was applied to observe the histological changes of rat endometrium;the microvessel density(MVD)in rat endometrium was detected by immunohistochemistry;the expressions of vascular endothelial growth factor(VEGF),TNF-α,IL-1β and IL-6 in rat serum were detected by ELISA;Western blot was applied to detect the expressions of VEGF and YAP proteins in rat endometrium.Results:Compared with sham operation group,the EM rats in model group showed obvious ectopic endometrial tissue lesions,increased number of interstitial cells,and obvious vascular proliferation,the weight,volume,MVD,the contents of VEGF,TNF-α,IL-1β,IL-6,and the expression levels of YAP,TAZ,and VEGF receptor 2(VEGFR2)proteins in endometrial tissue increased(P<0.05);compared with model group,the ectopic endometrium tissue of EM rats in positive drug group,Verteporfin group and low and high dose CEL groups gradually fell off,the number of interstitial cells were decreased,the structure was loose,and the blood vessels were significantly reduced,the weight,volume,MVD,the contents of VEGF,TNF-α,IL-1β,IL-6,and the expression levels of YAP,TAZ,and VEGFR2 proteins in endometrial tissue were decreased(P<0.05).Conclusion:CEL can inhibit angiogenesis in EM rats by inhibiting Hippo-YAP signal pathway.
