Study on effect of panax notoginseng saponins treatment in Th17/Treg cells differentiation balance through regulating mTORC1-HIF1α pathway
10.3969/j.issn.1000-484X.2024.11.012
- VernacularTitle:三七总皂苷调控mTORC1-HIF1α通路干预Th17/Treg分化平衡的作用研究
- Author:
Yujie BAO
1
;
Meiyu SHEN
;
Yuxi DI
;
Furong WANG
;
Lingling ZHOU
Author Information
1. 南京中医药大学药学院,南京 210023
- Keywords:
Panax notoginseng saponins;
Th17;
Treg;
mTORC1;
HIF1α
- From:
Chinese Journal of Immunology
2024;40(11):2310-2315
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effect of panax notoginseng saponins(PNS)on mTORC1-HIF1α signaling pathway,and to explore its effect and mechanisms on the differentiation balance of Th17/Treg cells in CD4+T cells.Methods:Isolate the spleens of C57BL/6 mice,then select CD4+T cells by magnetic beads and cultured in vitro.The optimal concentration of PNS was screened by the CCK-8,and then these cells were divided into control group and PNS treatment group(5,10 and 20 μg/ml),each gives correspond-ing drug treatment after 48 h.Afterwards,flow cytometry was used to detect differentiation of Th17/Treg cells.Real-time quantitative fluorescent PCR was used to detect the expressions of RORγt,Foxp3,mTOR,Raptor,HIF1α mRNA.ELISA was used to detect the levels of IL-17A and IL-10 in the supernatant of cell culture.Western blot was used to detect the expressions and phosphorylation levels of 4EBP1,S6K and HIF1α proteins.Results:5,10,20 μg/ml PNS could significantly inhibit Th17 cells differentiation and promote Treg cells differentiation;5,10,20 μg/ml PNS could significantly reduce the expression of RORγt mRNA,and then reduce the level of IL-17A;20 μg/ml PNS could significantly promote the expression of Foxp3 mRNA and increase the level of IL-10;10,20 μg/ml PNS could significantly decrease the phosphorylation of 4EBP1 and S6K;5,10,20 μg/ml PNS could significantly reduce the expression of HIF1α mRNA and inhibit the expression of HIF1α protein.Conclusion:Certain concentrations of PNS can inhibit the differentiation of Th17 cells in CD4+T cells,and promote the differentiation of Treg cells,which is related with modulating mTORC1-HIF1α signaling pathway.
