Effect of S100A10 macrophage-mediated inflammation and migration
10.3969/j.issn.1000-484X.2024.11.003
- VernacularTitle:S100A10对巨噬细胞介导的炎症及迁移的影响
- Author:
Jiaoyang LI
1
;
Shengnan LIU
;
Yuxin ZHAO
;
Jingtao GAO
;
Hui WANG
Author Information
1. 新乡医学院医学检验学院,新乡 453003
- Keywords:
Macrophages;
S100A10;
Inflammatory factor;
Migration
- From:
Chinese Journal of Immunology
2024;40(11):2257-2261
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of S100A10 macrophage-mediated inflammation and migration.Methods:C57BL/6J mouse model of S100A10-KO,and RAW264.7 cell line of S100A10-KO were established.Taking mice's peritoneal macro-phages(PMs)and bone marrow macrophages(BMDMs),resuscitating RAW264.7 cell lines,and collecting cells.qRT-PCR was used to detect the effect of S100A10 knockout on the secretion of inflammatory factors in macrophages;wound healing assay and Transwell assay were used to detect the effect of S100A10 knockout on macrophage migration;CCK8 kit was used to detect the effect of S100A10 knockout on the proliferation of RAW264.7 cells;qRT-PCR and Western blot were used to detect the expressions of matrix metallopro-teinase 9(MMP9)and non-muscle myosin heavy chain 9(MYH9).Results:After S100A10 knockout,the inflammatory factors IL-6,IL-1β and MCP-1 levels(P<0.05)secreted by RAW264.7,PMs and BMDMs were excreted decreased;cell scratches and Transwell showed that S100A10 knockout inhibited macrophage migration;CCK8 experiments showed that the proliferation capacity of macro-phages weakened after S100A10 knockout;qRT-PCR and Western blot experiments showed that the migration-related proteins MMP9 and MYH9 decreased after S100A10 knockout.Conclusion:S100A10 knockout decreases the secretion of inflammatory factors by macrophages and attenuated the migration and proliferation of macrophages.
