A novel homogeneous immunoassay technique——Quenchbody assay
10.3969/j.issn.1000-484X.2024.10.035
- VernacularTitle:新型均相免疫分析技术——荧光淬体免疫探针分析
- Author:
Guangwei ZHAO
1
,
2
;
Hao CHEN
;
Jinhua DONG
Author Information
1. 山东第二医科大学生命科学与技术学院,潍坊 261053
2. 山东大学基础医学院,济南 250012
- Keywords:
Antibody;
Immunoassay;
Fluorescence probe;
Quench;
Disease biomarker
- From:
Chinese Journal of Immunology
2024;40(10):2223-2228
- CountryChina
- Language:Chinese
-
Abstract:
Biochemical analysis is of great significance in many fields,such as disease diagnosis,environmental pollution source identification,food safety,etc.Immunoassay based on the reaction of antigen and antibody has the characteristics of high speci-ficity and diversity.Compared with other analytical methods such as liquid chromatography,which requires a large and expensive in-strument,immunodetection has the advantages of simplicity and good selectivity and is thus widely used in sample testing.Quench-body(Q-body)is a novel fluorescent immunosensor based on antigen-antibody reaction.It is constructed by labeling antibody fragment with fluorescent dye.Tryptophan in antibody leads to electron transfer and quenching of fluorescent,when it specifically binds with an-tigen,its fluorescence intensity recovers and presents concentration dependence.Therefore,the antigen concentration can be detected by detecting the change of fluorescence intensity of the Q-body.Q-body immunoassay is widely used in the rapid detection of environ-mental pollutants and disease markers due to its simple operation and high sensitivity,which is of great significance for environmental detection and disease diagnosis.In this paper,the principle,construction method,detection technology development and domestic and foreign research progress of Q-body are comprehensively discussed,and the future application of this technology is prospected.
