Association of MIF and MMPs gene interaction with ankylosing spondylitis and immunity
10.3969/j.issn.1000-484X.2024.10.022
- VernacularTitle:MIF、MMPs基因交互作用与强直性脊柱炎及免疫相关性研究
- Author:
Hui ZHU
1
;
Yongshen XU
Author Information
1. 河南科技大学第二附属医院,洛阳 471000
- Keywords:
MIF;
MMP;
GMDR;
Ankylosing spondylitis;
Immunity
- From:
Chinese Journal of Immunology
2024;40(10):2150-2157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the genetic susceptibility of MIF and MMPs family gene polymorphisms to ankylosing spon-dylitis(AS),as well as the correlation of gene-gene,gene-environment interactions in the pathogenesis of AS,and to find high risk factors of AS and screening of high-risk groups of AS.Methods:A total of 180 AS patients and 345 controls were included in this case-control study.Selected the tag SNPs of MIF and MMPs family genes(MIF rs2070767,MIF rs1007888,MMP1 rs498186,MMP2 rs243866,MMP3 rs591058,MMP8 rs11225395),then DNA was extracted and genotyped,and the relationship between genotype,allele distribution frequency,genetic model and the incidence of AS and the risk factors were further analyzed.Generalized multi-fac-tor dimensionality reduction method was used to analyze the correlation of the interaction between target genes and between genes and the environment and AS.The functional enrichment analysis and immune correlation analysis of MIF and MMPs were carried out.Re-sults:The genotype distribution frequency of the smallest alleles of MIF rs1007888,MMP1 rs498186,MMP8 rs11225395 were higher risks in AS group than in control group(P<0.05).GG genotype of MIFrs1007888,GG genotype of MMP1 rs498186,CT/TT genotype of MMP3 rs591058,and TT genotype of MMP8 rs11225395 were related to the risk of AS.Gene-gene interaction analysis found that MMP3 rs591058 and MMP8 rs11225395 interaction models were the best models.Gene-environment interaction analysis found that MIF rs2070767,MIF rs1007888,MMP1 rs498186,MMP2 rs243866,MMP3 rs591058,MMP8 rs11225395,smoking,drinking,and obesity interaction models were the best models.MIF and MMPs related genes were enriched in matrix metalloproteinases,macro-phage migration pathway and immune system regulation.Expression levels of MMPs were positively correlated with immune infiltra-tion.Conclusion:The single nucleotide polymorphisms of MIF and MMPs are related to the susceptibility to AS,and there is an inter-action between MIF and MMPs genes and the environment,which causes as through multiple biological processes,and may affect the process of as through immune infiltration.
