Changes and clinical significance of senescent cell proportion and senescence-associated cytokines in acute myeloid leukemia
10.3969/j.issn.1000-484X.2024.10.020
- VernacularTitle:衰老细胞比例及衰老相关细胞因子在急性髓系白血病中的变化及临床意义
- Author:
Linlin LIU
1
;
Qianshan TAO
;
Huiping WANG
;
Qing ZHANG
;
Zhimin ZHAI
Author Information
1. 安徽医科大学第二附属医院血液科,合肥 230601
- Keywords:
Acute myeloid leukemia;
Senescent cell;
Senescence-associated cytokines
- From:
Chinese Journal of Immunology
2024;40(10):2141-2145
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate changes and clinical significance of senescent cell proportion and senescence-associated cytokines in peripheral blood of patients with acute myeloid leukemia(AML)in different states.Methods:AML patients who were diagnosed and treated in the Second Hospital of Anhui Medical University were selected as research subjects(17 cases in newly diag-nosed group,16 cases in remission group,and 12 cases in relapse group),morphology of leukemia blasts and proportion of senescent cells in peripheral blood of patients in each group were observed by Regis and senescence specific β-galactosidase(SA-β-gal)staining,levels of senescence-associated cytokines IL-8,IL-1β,IL-6,IL-10 in peripheral blood of each group were detected by cytometric bead array(CBA),and differences and clinical significance among groups were compared.Results:Relapse group peripheral blood smear Regis staining showed that morphology of leukemia blasts increased significantly and proportion of SA-β-gal positive cells was significantly increased(P<0.01);levels of serum senescence-associated cytokines IL-8,IL-1β,IL-6 and IL-10 in relapse group were significantly increased(P<0.05);there was no correlation between serum senescence-associated cytokine levels and proportion of peripheral blood leukemia blasts(P>0.05);relapsed patients with higher IL-8 levels trend to have lower re-induction remission rates and worse overall survival after relapse(P<0.05);multivariate Cox regression analysis showed that IL-8 level was an indepen-dent risk factor for prognosis of relapsed patients(P<0.05).Conclusion:Increased proportion of senescent cells and significantly increased levels of senescence-associated cytokines IL-8,IL-1β,IL-6,IL-10 in peripheral blood of AML patients are closely related to disease recurrence,and relapsed patients with higher IL-8 level had poor prognosis.
