Role of oxidative stress and immune infiltration in idiopathic pulmonary fibrosis and study of Chinese medicine for prevention and treatment
10.3969/j.issn.1000-484X.2024.10.015
- VernacularTitle:氧化应激和免疫浸润在特发性肺纤维化中的作用及中药防治研究
- Author:
Yifeng XU
1
;
Shiwen KE
;
Keke LI
;
Zhaoqi YAN
;
Yueqi XU
;
Xiaogang XU
;
Yu WANG
;
Liangji LIU
Author Information
1. 江西中医药大学,南昌 330004
- Keywords:
Idiopathic pulmonary fibrosis;
Differentially expressed genes;
Central gene;
Oxidative stress;
Immune infiltration
- From:
Chinese Journal of Immunology
2024;40(10):2108-2115,中插5
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role and relationship between oxidative stress and immune infiltration in idiopathic pul-monary fibrosis(IPF),and to predict the relevant therapeutic herbal medicines and active ingredients.Methods:GSE10667 gene expression profiles were downloaded from GEO database to obtain differential expression genes,differential expression of oxidative stress genes(DEOSGs)were identified in combination with oxidative stress genes.GSEA was used to evaluate the pathways and biologi-cal processes in IPF,and GO,KEGG and PPI network analysis were performed on DEOSGs.Candidate central genes were derived from PPI results and CytoHubba,and GSE110147 was validated as an independent group to identify central genes;in addition,the immune microenvironment of samples was evaluated using CIBERSORTF,and correlation between central gene levels and relative proportion of immune cells was explored;finally,therapeutic herbal medicines and components were predicted by central genes,and mole-cular docking verification was carried out.Results:A total of 51 DEOSGs,four central genes(ICAM-1,APOE,MMP-1,TGF-β2)were obtained;DEOSGs were mainly related to oxidative stress,immune response,etc;four central gene levels were closely correlated with 8 relative proportions of immune cells;therapeutic herbal medicines included 4 flavors such as Huangqi and Chuanxiong,and the active ingredients included 8 kinds of β-carotene,etc,the molecular docking results were stable.Conclusion:Oxidative stress and immune firing are exist in IPF,and oxidative stress may be recognized by immune cells or directly activate immune cells.
