Bioinformatics and transcriptome sequencing analysis of differential expression profile of angiosarcoma cells and angiosarcoma stem-like cells derived exosomes
10.3969/j.issn.1000-484X.2024.10.011
- VernacularTitle:基于生物信息学和转录组测序分析血管肉瘤细胞和血管肉瘤干细胞样细胞来源外泌体差异表达谱
- Author:
Kai ZHAO
1
;
Wenping CAI
;
Hao PENG
;
Shan JIN
;
Lijuan PANG
Author Information
1. 石河子大学医学院第一附属医院病理科,石河子大学医学院病理学系,新疆地方与民族高发病教育部重点实验室,石河子 832002
- Keywords:
Angiosarcoma;
Cancer stem cells;
Exosomes;
Transcriptome sequencing
- From:
Chinese Journal of Immunology
2024;40(10):2083-2090,中插1-中插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate key differential expressed genes(DEGs),enriched biological functions and signaling pathways in exosomes derived from angiosarcoma cells ISOHAS and angiosarcoma stem-like cells Sphere by bioinformatics analysis,to provide new therapeutic targets for angiosarcoma.Methods:Transcriptome sequencing of exosomes derived from ISOHAS and Sphere were performed to screen for DEGs by|log2FC|>2 and FDR<0.05 as criteria.Bioinformatics analysis was used to enrich GO and KEGG pathways of DEGs to identify biological functions and signaling pathways of enriched DEGs.STRING database was used to screen key DEGs,and iPath was used to visualize to identify metabolic pathways enriched by DEGs.Results:Transcriptome sequencing results showed that 91 DEGs were identified in exosomes derived from ISOHAS and Sphere.GO and KEGG pathway enrichment analysis demonstrated that main biological functions and signaling pathways enriched by DEGs were response to glucocorticoid and TNF signaling pathway,respectively.STRING database demonstrated that TNF and IL-6 were key DEGs.iPath metabolic network analysis demonstrated that DEGs were mainly identified in lipid metabolism and nucleotide metabolism.Conclusion:Sphere-derived exosomes may influence occurrence and development of angiosarcoma by carrying key genes TNF and IL-6 to interfere with glucocorticoid response,TNF signaling pathway,lipid metabolism,nucleotide metabolism and other biological functions and signaling pathways,providing new ideas for therapeutic targets for angiosarcoma.
