Denatonium benzoate promotes MrgprB2-mediated rat mast cell degranulation
10.3969/j.issn.1000-484X.2024.10.003
- VernacularTitle:苦精促进MrgprB2介导的鼠肥大细胞脱颗粒
- Author:
Huaping XU
1
;
Xiaoyun SHI
;
Jiexin ZOU
;
Xin LI
;
Mengting XIE
;
Shiyu XIAO
;
Linbo SHI
Author Information
1. 南昌大学第一附属医院,南昌 330006
- Keywords:
Denatonium benzoate;
MrgprB2;
Mast cell degranulation;
Ca2+mobilization
- From:
Chinese Journal of Immunology
2024;40(10):2037-2041
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potent effects of denatonium benzoate(DB)on Mas-related G protein-coupled receptor-B2(MrgprB2)-mediated rat mast cell degranulation.Methods:RBL-2H3 cells were treated with DB overnight,before challenged with MrgprB2 ligands substance P(SP).The release of β-hex from MrgprB2-activated RBL-2H3 was detected by substrate method.Detec-tion of LTC4,IL-6,TNF-α and cPLA2 activity were performed by ELISA.The Ca2+influx and the expression of RBL-2H3 MrgprB2 re-ceptors were measured by fluorescence assay.Results:The results showed 10 μmol/L,50 μmol/L,80 μmol/L,100 μmol/L DB treat-ments promoted β-hex and LTC4 releases from activated RBL-2H3,accompanied by increased Ca2+mobilization and cPLA2 activa-tion.DB treatments did not affect IL-6 and TNF-α LTC4 releases in MrgprB2-activated RBL-2H3,as well as the levels of MrgprB2 ex-pression in mast cells.Conclusion:Taken together,DB enhanced the MrgprB2-mediated RBL-2H3 degranulation in vitro,probably by up-regulating Ca2+mobilization in activated cells.
