Local immune microenvironment analysis of sodium dodecyl sulfate-induced irritant contact dermatitis model in mice
10.3969/j.issn.1000-484X.2024.10.002
- VernacularTitle:十二烷基硫酸钠诱导的小鼠刺激性接触性皮炎及局部免疫微环境分析
- Author:
Yueling ZENG
1
;
Limin FAN
;
Huifang CHEN
;
Ying HE
;
Ailin TAO
;
Xueting LIU
Author Information
1. 广州医科大学附属第二医院,广东省过敏反应与免疫重点实验室,呼吸疾病国家重点实验室变态反应研究室,广州 510260
- Keywords:
Irritant contact dermatitis;
Endotype;
Neutrophil;
Type 17 inflammation;
IL-17A
- From:
Chinese Journal of Immunology
2024;40(10):2031-2036
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an irritant contact dermatitis(ICD)model induced by sodium dodecyl sulfate(SDS)in mice,and explore its endotype to provide an experimental and theoretical basis for subsequent precise treatment.Methods:Mice were randomly divided into two groups(model group and control group),4%SDS was topically applied to induce ICD in mice,saline was used on control group,the dose and frequency were consistent with model group,and the skin lesions of mice were observed.Epidermal thickness and inflammatory cell infiltration were analyzed by HE staining,toluidine blue staining and immunofluorescence staining.Real-time quantitative PCR was performed to investigate mRNA expression levels of cytokines.Results:Compared with control group,mice in ICD model group showed epidermal thickness on the back of neck,and the numbers of inflammatory cells were increased in dermis.The number of neutrophils,macrophages and T cells were increased.Expressions of Il17a and Il17f mRNA levels were increased.Conclusion:SDS-induced ICD model is successfully established,with the elevated infiltration of neutrophils,macrophages and T cells,and secretion of type 17 cytokines.
