Development of multiplex cytokine detection reagents and its application in myeloma
10.3969/j.issn.1000-484X.2024.09.025
- VernacularTitle:多重细胞因子检测试剂的开发及其在骨髓瘤中的应用
- Author:
Huoying PENG
1
,
2
;
Zhiyao ZHANG
;
Xiangjun ZHENG
;
Peng WEI
;
Di HU
;
Wenming CHEN
;
Xiaobo YU
Author Information
1. 青岛大学基础医学院,青岛 266071
2. 国家蛋白质科学中心-北京(凤凰中心),医学蛋白质组全国重点实验室,北京蛋白质组研究中心,北京 102206
- Keywords:
Multiplex bead-based immunoassay by flow cytometry;
Relapse/refractory multiple myeloma;
Cytokine;
Angiogen-esis;
Bone remodeling
- From:
Chinese Journal of Immunology
2024;40(9):1944-1950
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop multiplex cytokine detection reagents to analyze expression levels of cytokines,angiogene-sis and bone remodeling proteins in relapse/refractory multiple myeloma(RRMM).Methods:Multiplex bead-based immunoassay by flow cytometry was used to develop quantitative detection reagents of multiplex cytokines,which were applied to detect serum samples from 55 RRMM patients and 22 healthy controls.Expression levels of cytokines,angiogenesis,and bone remodeling proteins in pa-tients,and their correlation with clinical characteristics were analyzed.Results:Detection reagents of 10-plex cytokine immunoassay were successfully developed in this study,with average sensitivity of 7.1 pg/ml,average recovery rate of 97.4%,average intra-assay CV of 4.8%,and average inter-assay CV of 9.0%.In addition,results of RRMM samples found that levels of IL-2,IL-17,DKK1,RANKL and OPG were positively correlated with the level of IgG monoclonal protein,and TIMP1 was positively correlated with levels of IgG and IgA monoclonal protein.Conclusion:In this study,ten kinds of cytokine detection reagents with high sensitivity and speci-ficity are developed,and we found that IL-2,IL-17,DKK1,RANKL,OPG and TIMP1 have potential value in tracking disease pro-gression in RRMM.The established development process of multiplex cytokine reagents has important reference significance for ex-panding the development and application of multiplex detection reagents for protein markers in the future.
