Effect of miR-1298-5p on biological behavior of non-small cell lung cancer cells and tumor immune microenvironment by targeting MSH2 gene
10.3969/j.issn.1000-484X.2024.09.016
- VernacularTitle:miR-1298-5p通过靶向MSH2基因对非小细胞肺癌细胞生物学行为及肿瘤免疫微环境的影响
- Author:
Yaosheng ZHANG
1
;
Xiuli YANG
;
Xiao REN
;
Hongli WANG
;
Ling SHEN
;
Guosheng HUANG
Author Information
1. 南阳医学高等专科学校第一附属医院肿瘤内科,南阳 473000
- Keywords:
miR-1298-5p;
MSH2;
Non-small cell lung cancer
- From:
Chinese Journal of Immunology
2024;40(9):1889-1894,1901
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential mechanism of miR-1298-5p in non-small cell lung cancer(NSCLC)to regu-late the MSH2 gene and its effect on the biological behavior of tumor cells and the tumor immune microenvironment.Methods:Bioin-formatics was used to identify the key genes and miRNA involved in NSCLC.Cell proliferation was detected by CCK-8 assay,and cell invasion and migration were detected by Transwell assay.Levels of inflammatory factors were detected by ELISA assay.Western blot was used to measure the expression of MSH2 in cells,and fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expressions of miR-1298-5p and MSH2 gene in NSCLC cells.Dual luciferase reporter gene assay was performed to verify the targeting relationship between miR-1298-5p and MSH2.Spearman correlation analysis was performed to correlate miR-1298-5p with immune cells and immune factors in the tumor immune microenvironment.Results:The level of miR-1298-5p was down-regulated in NSCLC cells compared with normal lung tissue cells.miR-1298-5p overexpression inhibited the proliferation,migration and inva-sion of NSCLC cells.MSH2 was confirmed to be a target gene of miR-1298-5p using luciferase reporter gene assay.Furthermore,down-regulation of miR-1298-5p in NSCLC cells could be reversed by silencing MSH2.miR-1298-5p expression levels were negatively corre-lated with the levels of Treg,IL-10,and TGF-β,and positively correlated with the levels of CD3+T,CD4+T,CD8+T,NK cells,IL-2,and IFN-γ.Conclusion:miR-1298-5p negatively regulates MSH2 to inhibit the proliferation,invasion and migration of NSCLC cells and improve the tumor immune microenvironment.
