Fasudil inhibits Aβ1-42-induced microglial inflammatory response by inhibiting activation of NLRP3 inflammasome
10.3969/j.issn.1000-484X.2024.09.007
- VernacularTitle:法舒地尔通过抑制NLRP3炎症小体激活抑制Aβ1-42诱导的小胶质细胞炎症反应
- Author:
Minfang GUO
1
;
Peijun ZHANG
;
Jingwen YU
;
Tao MENG
;
Yanhua LI
;
Na LI
;
Mengdi LI
;
Yulu LI
;
Lijuan SONG
;
Jiezhong YU
;
Cungen MA
Author Information
1. 山西大同大学脑科学研究所,大同 037009
- Keywords:
Fasudil;
Microglia;
NLRP3 inflammasome;
Inflammatory reaction
- From:
Chinese Journal of Immunology
2024;40(9):1833-1837
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore mechanism of Fasudil reducing Aβ1-42 induced BV2 cell injury based on NLRP3 inflamma-some.Methods:BV2 cells were divided into:normal control group,Aβ stimulation group,Aβ+Fasudil intervention group,Aβ+MCC950(NLRP3 inhibitor)intervention group.Cell morphology was observed under microscope.Cell activity was determined of by CCK8.NO release was measured by Griess.NLRP3,caspase 1 and IL-18 expressions were detected by immunofluorescence staining.NLRP3,ASC,caspase 1,IL-1β and IL-18 expressions were detected by Western blot.Results:Compared with normal control group,BV2 cells in Aβ stimulation group were activated and showed amoeba-like shape,cell activity was decreased,NO production was increased,NLRP3,ASC,caspase 1,IL-1β and IL-18 expressions were increased.Fasudil intervention and MCC950 intervention inhibited cell injury induced by Aβ1-42 in which BV2 cell morphology tended to be normal,cell activity was increased,while produc-tion of NO was reduced,and NLRP3,ASC,caspase 1,IL-1β and IL-18 expressions were down-regulated,there was no significant difference between Fasudil intervention group and MCC950 intervention group.Conclusion:Fasudil may alleviate Aβ1-42 induced BV2 cell injury and inflammatory reaction by inhibiting NLRP3 inflammasome activation.
