Methodological investigation of cyclophosphamide-induced immunocompro-mised mice model and effect of extract of Rosa laevigata Michx.combined with probiotics on immune function
10.3969/j.issn.1000-484X.2024.08.021
- VernacularTitle:环磷酰胺诱导的小鼠免疫低下模型的方法学考察及金樱子提取物联合益生菌调节免疫功能的研究
- Author:
Zekun LIU
1
;
Junli MA
;
Yan LI
;
Ningning ZHENG
;
Lili SHENG
;
Houkai LI
Author Information
1. 上海中医药大学中药学院,上海 201203
- Keywords:
Immunocompromised;
Cyclophosphamide;
Animal models;
Drug evaluation
- From:
Chinese Journal of Immunology
2024;40(8):1701-1708
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a mice model that is more in line with the human immunocompromised state,and to inves-tigate the regulatory effect of Rosa laevigata Michx.,probiotics and their combination on their immune function.Methods:Immunosup-pressive model of mice was established by using cyclophosphamide with different dosage,time and end point,and compared with im-munosuppressive model induced by hydrocortisone and cyclosporine A,immunosuppressive model of mice was established by injecting 25 mg/kg cyclophosphamide 7 times every other day,without recovery period;changes in the proportion of lymphocytes in spleen(CD4+T cells,CD8+T cells,CD19+B cells)were analyzed by flow cytometry;qRT-PCR was used to detect expression levels of inflam-matory factors(IL-1β and IL-6)mRNA in spleen.Results:After continuous injection of 80 mg/kg cyclophosphamide for 3 days,the thymus was atrophied,the spleen was significantly swelling,proportion of immune cells CD4+T cells,CD8+T cells and CD19+B cells in spleen were significantly decreased,while mRNA expressions of IL-1β and IL-6 were up-regulated.After 7 injections of 25 mg/kg cyclophosphamide every other day,the thymus and the spleen were significantly atrophied,proportion of CD4+T cells and CD8+T cells in spleen showed an increasing trend,and mRNA expressions of IL-1β and IL-6 were up-regulated;after 1 week or 2 weeks recovery period from cyclophosphamide injection,weight and index of immune organs,proportion of spleen lymphocytes and mRNA expres-sions of inflammatory cytokines in spleen were significantly reversed;Rosa laevigata Michx.combined with probiotics could signifi-cantly reverse the up-regulation of CD4+T cells and inflammatory factor IL-1β caused by cyclophosphamide.Conclusion:In this study,an immunocompromised mice model has been established with 7 injections of 25 mg/kg cyclophosphamide every other day and without recovery period.And a certain positive immunomodulatory effect of the combination of Rosa laevigata Michx.and probiotics has been found.
