Yeast β-glucan vehicle based oral OVA vaccine(WGP-OVA vaccine)induces humoral and antigen-specific T cell immune responses
10.3969/j.issn.1000-484X.2024.08.002
- VernacularTitle:基于酵母β-葡聚糖载体的口服OVA疫苗(WGP-OVA疫苗)诱导体液免疫及抗原特异性T细胞免疫反应
- Author:
Yu BAI
1
;
Chunjian QI
;
Lei XIA
;
Shuyan HE
;
Liuyang HE
Author Information
1. 南京医科大学附属常州市第二人民医院中心实验室,常州 213000
- Keywords:
Whole β-glucan particles;
Ovalbumin;
Oral vaccine;
Immunoglobulins;
Antigen-specific T cells
- From:
Chinese Journal of Immunology
2024;40(8):1579-1583,1589
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate humoral and antigen-specific T cell immune responses induced by oral administration of WGP-OVA in C57BL/6 mice.Methods:C57BL/6 mice were orally administered with PBS,WGP and WGP-OVA for 17 days,respec-tively.The serum immunoglobulin levels including IgG1,IgG2a,IgG3 and IgM of mice were detected using LEGENDplexTM Multi-An-alyte Flow Assay Kit.HE staining was used to compare the size of lymphoid nodules in spleens.FACS was used to analyze the percent-age of F4/80+macrophages and F4/80+SIINFEKL+macrophages in inguinal lymph nodes(ILNs).In addition,the T cell-mediated im-munity stimulated by the WGP-OVA oral vaccine was analyzed by ILNs-infiltrating antigen-specific CD8+T cell using MHC Tetramer assay.Results:Oral administration of WGP-OVA significantly increased the contents of IgG1,IgG2a,IgG3 and IgM in the serum,and enlarged the lymphoid nodules in spleens,compared to those orally administered with PBS.Oral administration of WGP-OVA pro-moted the ILNs-infiltration of F4/80+macrophages,and enhanced the antigen-presentation of OVA in macrophages in ILNs.Moreover,the expansion of OVA specific CD8+CTLs in ILNs was also stimulated by WGP-OVA.Conclusion:WGP-OVA involved in both the humoral and antigen-specific T cell immune responses in C57BL/6 mice.
