Preparation and in vitro evaluation of polyethylene glycol derivatives modified IL-12

VernacularTitle:聚乙二醇衍生物修饰的IL-12制备及体外评价
Author: Yi LIU ^{1
,

2} ; Zhenyang ZOU ; Zongyan LI ; Feng HAN ; Tiankui QIAO ; Jiangang WANG
Author Information

1. 中国海洋大学医药学院,青岛 266000
2. 康立泰生物医药(青岛)有限公司,青岛 266000
Keywords: PEG; IL-12; Hemogram recovery; Anti-tumor
From: Chinese Journal of Immunology 2024;40(7):1495-1500,1506
CountryChina
Language:Chinese
Abstract: Objective:To prepare IL-12 modified with polyethylene glycol derivatives and determine its modification sites,and to evaluate it in vitro in terms of stability,hemogram recovery,anti-tumor effects and other aspects.Methods:IL-12 was modified with polyethylene glycol propionaldehyde and maleimide imide derivatives,and modified sites of two different derivatives were deter-mined by ultra performance liquid chromatography(UPLC).Recombinant human IL-12 and PEG-IL-12 were used to stimulate NK92 cells,activity and cytotoxicity of IFN-γ were evaluated by kits.CD34+was stimulated to evaluate its blood picture recovery potential.NK cell killing was stimulated to evaluate its effectiveness in improving immunity and anti-tumor effects.Results:Compared with recombinant human IL-12,PEG-IL-12 had higher stability,blood picture recovery potential and anti-tumor effects.Conclusion:PEG-IL-12 can effectively overcome many drawbacks of recombinant human IL-12,greatly improving possibility of its widespread applica-tion in clinical trials.