Correlation between peripheral blood Siglec-7+monocytes and Th1/Th2 cells in patients with brucellosis
10.3969/j.issn.1000-484X.2024.07.021
- VernacularTitle:布鲁氏菌病患者外周血中Siglec-7+单核细胞与Th1/Th2细胞的相关性研究
- Author:
Yueyue HE
1
;
Yuejie ZHU
;
Quan WANG
;
Fengbo ZHANG
;
Jianbing DING
Author Information
1. 新疆医科大学基础医学院,乌鲁木齐 830011
- Keywords:
Brucellosis;
Siglec-7;
Monocytes;
Th1/Th2 cells;
Immune escape
- From:
Chinese Journal of Immunology
2024;40(7):1474-1478
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore expression of Siglec-7 on monocytes in peripheral blood of patients with brucellosis and cor-relation between Siglec-7+monocytes and Th1/Th2 cells,and to analyze clinical significance of Siglec-7 molecule in patients with bru-cellosis.Methods:Fifty patients with newly diagnosed Brucella infection(BI group)and 46 healthy controls(control group)were in-cluded.Flow cytometry was used to detect expression of Siglec-7 on monocytes,and correlation between Siglec-7+monocytes and clini-cal indicators of patients with brucellosis were analyzed.Flow cytometry was used to detect Th1/Th2 cells levels,and CBA method was used to detect IFN-γ and IL-4 in peripheral serum.Correlation between Siglec-7+monocytes and Th1/Th2 cells,IFN-γ/IL-4 were ana-lyzed.Results:Siglec-7+monocytes level in BI group was significantly higher than that in control group(P<0.001);Siglec-7+mono-cytes level in patients with abnormal liver function and lung X-ray were higher than those in patients with normal liver function and lung X-ray(P<0.001,P<0.05);Compared with control group,Th2 cell and IL-4 levels were significantly increased(P<0.05),while Th1 cells and IFN-γ levels were significantly reduced(P<0.001);Th1/Th2 and IFN-γ/IL-4 were also significantly reduced(P<0.001).Siglec-7+monocyte level was negatively correlated with Th1 and IFN-γ(r=-0.651,r=-0.407),while was positively correlated with Th2 and IL-4(r=0.706,r=0.530).Conclusion:During Brucella infection,increased percentage of Siglec-7+monocytes may be involved in Th1/Th2 cell imbalance.
