Bioinformatics-based gene set enrichment and immune cell infiltration analysis of chronic spontaneous urticaria based on GEO database
10.3969/j.issn.1000-484X.2024.07.010
- VernacularTitle:基于GEO数据库的慢性自发性荨麻疹基因集富集及免疫细胞浸润分析
- Author:
Lu HAN
1
;
Yang ZHOU
;
Yue WAN
;
Ning GUAN
;
Runan FANG
;
Jianhong LI
Author Information
1. 北京中医药大学东直门医院,北京 100700
- Keywords:
Chronic spontaneous urticaria;
Gene set enrichment analysis;
Immune cell infiltration;
Coagulation cascade reac-tion;
Autoimmunity;
Th1/Th2 cells
- From:
Chinese Journal of Immunology
2024;40(7):1405-1410,中插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on gene expression omnibus(GEO),differential expressed genes,gene set enrichment analysis(GSEA)and immune cell infiltration analysis were performed on microarray data of chronic spontaneous urticaria(CSU)expression profile,to gain more insight into the pathogenesis of CSU.Methods:The GSE72541 raw data were obtained from the GEO.Differential expressed genes were screened using R software.String database were used to construct the the protein-protein interaction(PPI)net-work.Gene ontology(GO)and Kyoto encyclopedia of gene and genomes(KEGG)enrichment analysis were performed using GSEA software.The ssGSEA method was used to analyze the infiltration of immune cells in the expression profile.Results:Genes closely related to platelet activation and its function were up-regulated in CSU serum,while genes related to Th1 cell chemotaxis were down-regulated in CSU serum.Biological processes and signal pathways related to coagulation cascade reaction,regulation of vascular per-meability,immune and inflammatory reactions,and mood-modulating were up-regulated in CSU group.Immunized cell infiltration analysis showed that activated B cells,immature B cells,follicular helper T cells,and Th2 cells were down-regulated in the CSU serum.Conclusion:Platelet activation,coagulation cascade reaction and the imbalance of Th1/Th2 immunity play important roles in the pathogenesis of CSU.
